Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 37RQ
Summary Introduction
To review:
The evidence used by Watson and Crick to construct the Deoxyribose
Introduction:
The structure of DNA is composed of two antiparallel strands of the polynucleotide chain. The backbone is composed of sugar–phosphate bonds. The nitrogenous bases, which are adenine, thymine, cytosin, end guanin, ere stacked in the center. They are linked to the backbone by the phosphodiester bonds. The number of purines equals the number of pyrimidines. There are three major forms of DNA, namely A-DNA, B-DN, And Z-DNA. The most common form of DNA is B-DNA.
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Enzymes isolated from bacteria that cut DNA at specificsequences are called ____________________.
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Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
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- The eukaryotic mRNA is protected from degradation by a 3′ _____________.arrow_forwardThe average of a number of closely related but nonidentical sequences is referred to as a(n) _____________.arrow_forwardQuestion 49 The Sanger method of DNA sequencing follows the principle of complementarity just like in the replication process. A) True B) Falsearrow_forward
- If Adenine was added where Guanine should have been added, this would be an example of a ___________________ mutation.arrow_forwardQuestion 1. Enzymes, proteins and deoxyribonucleic acid (DNA) macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA repreduction. are important biological a) Compare the process of protein synthesis between eukaryote mRNA and viral RNA b) With the aid of a diagram, draw an adapter molecule that recognizes the codons of mRNA and explain its functions in DNA translation.arrow_forwardFour basic properties of the genetic code are specificity, degenerate, almost universal, and _____________.arrow_forward
- If a base was added or deleted and the reading frame shifted, this would be an example of a ___________________ mutation.arrow_forwardQuestion #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…arrow_forwardTransposable elements are short segments of DNA, presentin ___________ locations, that move around the genomearrow_forward
- Transcription is the synthesis of _________, using _________ as a template.arrow_forwardQUESTION NO. 1 Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. The study of such patients has enhanced our knowledge of DNA repair because XP is caused by defective DNA repair nucleotide excision repair. (A variant, XP-V, is deficient in postreplication repair.) In nucleotide excision repair A. removal of the damaged bases occurs on only one strand of the DNA. B. only thymine dimers generated by UV light can be removed . C. the excision nuclease is an exonuclease. D. a single multifunctional enzyme carries out the repair process. E. only the damaged nucleotides are removed. QUESTION NO.2 Homologous recombination: A. occurs only between two segments from the same DNA molecule. B. requires that a specific DNA sequence be present. C. requires one of the duplexes undergoing recombination be nicked in both strands. D. involves a…arrow_forwardQuestion 43 The addition of restriction endonucleases in the cloning process is done following the ligation with DNA ligase. A) True B) Falsearrow_forward
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