Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 19RQ
Summary Introduction
To review:
The role of promoters, enhancers, silencers, and insulators ingene expression.
Introduction:
Gene expression is the process of expression or encoding of the gene so that it canbe transcribed and translated to form functional gene products. There are various regulatory DNA (deoxyribonucleic acid) sequences thatplay a role in controlling (inhibiting or stimulating) the gene expression levels.
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Gene expression is initiated largely in response to changes in the ____________________________ in prokaryotes as opposed to changes affecting the homeostasis of the whole organism in eukaryotes.
QUESTION 8
Consider the pathway for the synthesis of the amino acid arginine in Neurospora:
ARG-E
→ citrulline
ARG-F
ARG-H
ornithine
argininosuccinate arginine
Mutant strains of Neurospora may carry one or more mutations. Neurospora mutant strain b is grown on minimal media plus supplements as shown.
Growth is shown by (+) and no growth is shown by (o).
Supplements
ornithine
mutant nothing
citrulline
arginino-
succinate
arginine
strain
What can you conclude from these data?
O Strain a has only one mutation and it is in ARG-E.
O Strain b has only one mutation and it is in ARG-H.
O Strain a has a mutation in ARG-F and strain b has a mutation in ARG-E.
O Strain b has mutations in ARG-E, ARG-F, and ARG-H.
+ +
QUESTION 19
Place the following steps in the expression of the lac operon in the order in which each occurs for the first time after a cell is induced.
v A phosphodiester bond is formed between two ribonucleotides.
- A peptide bond is formed between the first two amino acids in beta-galactosidase (lac Z).
Sigma protein binds to a specific DNA sequence.
RNA polymerase ends transcription of the lacA gene.
v A protein leaves an operator DNA sequence.
v A ribosomal RNA binds to a complementary sequence in mRNA.
Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
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