Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 32RQ
Summary Introduction
To review:
Thesource of protons, which are detected by the ion sensor in the ion torrent deoxyribose
Introduction:
The DNA contains the genetic information in the form of nitrogenous bases, which are adenine, thymine, cytosine, and guanine. This coding can be deciphered by performing the sequencing of codes of the DNA. There are various methods by which DNA sequencing can be performed such as Sanger’s chain termination method, chemical method, automated sequencing, and ion torrent DNA sequencing.
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Question # 26: You are working with a genetic construct of your favorite protein,
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B) Near the ladder marker for 300 Da
C) None of the other answers is correct
D) Near the ladder marker for 300 kDa
O Near the ladder marker for 33 kDa
IDA= loo0,kba
X ko
Question #27: Which of the following methods would be most appropriate to
determine high-resolution, atom-level structural information about a relatively
small, difficult-to-crystallize protein with interesting dynamics?
ANMR spectroscopy
X-ray crystallography
Cryo-electron microscopy
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a SDS-PAGE
Multisubunit proteins in which some or all of the subunitsare identical are referred to as _______________________.
Enzymes isolated from bacteria that cut DNA at specificsequences are called ____________________.
Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 17 - Prob. 1QCh. 17 - Prob. 2QCh. 17 - Prob. 3QCh. 17 - Prob. 4QCh. 17 - Prob. 5QCh. 17 - Prob. 6QCh. 17 - Prob. 7QCh. 17 - Prob. 8QCh. 17 - Prob. 1RQCh. 17 - Prob. 2RQ
Ch. 17 - Prob. 3RQCh. 17 - Prob. 4RQCh. 17 - Prob. 5RQCh. 17 - Prob. 6RQCh. 17 - Prob. 7RQCh. 17 - Prob. 8RQCh. 17 - Prob. 9RQCh. 17 - Prob. 10RQCh. 17 - Prob. 11RQCh. 17 - Prob. 12RQCh. 17 - Prob. 13RQCh. 17 - Prob. 14RQCh. 17 - Prob. 15RQCh. 17 - Prob. 16RQCh. 17 - Prob. 17RQCh. 17 - Prob. 18RQCh. 17 - Prob. 19RQCh. 17 - Prob. 20RQCh. 17 - Prob. 21RQCh. 17 - Prob. 22RQCh. 17 - Prob. 23RQCh. 17 - Prob. 24RQCh. 17 - Prob. 25RQCh. 17 - Prob. 26RQCh. 17 - Prob. 27RQCh. 17 - Prob. 28RQCh. 17 - Prob. 29RQCh. 17 - Prob. 30RQCh. 17 - Prob. 31RQCh. 17 - Prob. 32RQCh. 17 - Prob. 33RQCh. 17 - Prob. 34RQCh. 17 - Prob. 35RQCh. 17 - Prob. 36RQCh. 17 - Prob. 37RQCh. 17 - Prob. 38RQCh. 17 - Prob. 39RQCh. 17 - Prob. 40RQCh. 17 - Prob. 41FBCh. 17 - Prob. 42FBCh. 17 - Prob. 43FBCh. 17 - Prob. 44FBCh. 17 - Prob. 45FBCh. 17 - Prob. 46FBCh. 17 - Prob. 47FBCh. 17 - Prob. 48FBCh. 17 - Prob. 49FBCh. 17 - Prob. 50FBCh. 17 - Prob. 51SACh. 17 - Prob. 52SACh. 17 - Prob. 53SACh. 17 - Prob. 54SACh. 17 - Prob. 55SACh. 17 - Prob. 56TQCh. 17 - Prob. 57TQCh. 17 - Prob. 58TQCh. 17 - Prob. 59TQCh. 17 - Prob. 60TQCh. 17 - Prob. 61TQCh. 17 - Prob. 62TQCh. 17 - Prob. 63TQCh. 17 - Prob. 64TQCh. 17 - Prob. 65TQCh. 17 - Prob. 66TQCh. 17 - Prob. 67TQCh. 17 - Prob. 68TQCh. 17 - Prob. 69TQCh. 17 - Prob. 70TQCh. 17 - Prob. 71TQCh. 17 - Prob. 72TQCh. 17 - Prob. 73TQ
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- Question 1. Enzymes, proteins and deoxyribonucleic acid (DNA) macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA repreduction. are important biological a) Compare the process of protein synthesis between eukaryote mRNA and viral RNA b) With the aid of a diagram, draw an adapter molecule that recognizes the codons of mRNA and explain its functions in DNA translation.arrow_forwardThe _____________ is the term used for polypeptide destruction motifs.arrow_forwardQuestion 2. Ribosomes are cellular structures that are composed of protein and RNA; this structure is responsible for catalyzing peptide bond formation between amino acids during a process known as translation. a) Many antibiotics that kill bacteria target translation. Why might this be an effective mechanism to kill bacteria? Why don't antibiotics also kill human (eukaryotic) ribosomes? b) The antibiotic Kasugamycin (KSG) destabilizes the P-site of the ribosome. Describe what parts of translation would be altered in the presence of this antibiotic. c) How does the following graph show the efficacy of translational knockdown with KSG? Met-Methionine C % of Met incorporation 100 80 60 40 20 0 + 0 2 4 6 8 KSG concentration (mg/ml) 10arrow_forward
- Sequence changes in DNA are called ______________.arrow_forwardA base change that has no discernible effect is called a____________________ mutation.arrow_forwardWhen nitrogenous bases in single-stranded polynucleotides are incorporated into doublestranded molecules, base pair formation results in altered UV light absorption. This is called the _____________ effectarrow_forward
- Question 45 When TRNAS and rRNAs have bases that H-bonds with other bases far apart from each other, the RNA molecules assume its secondary and tertiary structure. A) True B) Falsearrow_forwardQuestion 6 The helical turns of the DNA does not only provide spaces for binding with regulatory proteins but they cause the molecule to resemble a spiral staircase. A) True B) Falsearrow_forwardQUESTION NO. 1 Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. The study of such patients has enhanced our knowledge of DNA repair because XP is caused by defective DNA repair nucleotide excision repair. (A variant, XP-V, is deficient in postreplication repair.) In nucleotide excision repair A. removal of the damaged bases occurs on only one strand of the DNA. B. only thymine dimers generated by UV light can be removed . C. the excision nuclease is an exonuclease. D. a single multifunctional enzyme carries out the repair process. E. only the damaged nucleotides are removed. QUESTION NO.2 Homologous recombination: A. occurs only between two segments from the same DNA molecule. B. requires that a specific DNA sequence be present. C. requires one of the duplexes undergoing recombination be nicked in both strands. D. involves a…arrow_forward
- A posttranslational modification that directs a polypeptide to a specific location is called a _____________ modification.arrow_forwardQuestion 1.)Draw the main chain structure of a parallel beta sheet that has 4 amino acids in one strand and 5 in the other. Show all of the backbone atoms.Write R1, R2, R3, etc, instead of drawing side chains with the following exception: draw one proline in its entiretywith its side chain. You can put it anywhere in your sheet – I will find it. Add in all hydrogen bonds that stabilize this sheet using dashed lines.arrow_forwardThe eukaryotic mRNA is protected from degradation by a 3′ _____________.arrow_forward
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