Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 21RQ
Summary Introduction
To review:
The total number of base pairs and the length of the deoxyribose
Introduction:
The DNA is the inherited material of the cells. It is a double-stranded polynucleotide comprising deoxyribose sugar and nitrogenous basesthat are adenine (A), thymine (T), guanine (G), and cytosine (C). In a cell, DNA is compacted in the form of chromosomes. It is found as finethreads thatcoil around themselves to form a helical structure.
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Question 20 : The restriction map of the plasmid pSC48 is presented below. The numbers in
parentheses correspond to the position of the respective restriction site with respect to
position (0).
(0)
BamHI
ЕcoRI
(4640)
(620)
PSC48
5200 pb
BamHI
(1130)
Smal
ЕcoRI
(3420)
(1350)
The plasmid has been digested by one or several restriction enzyme(s). The digestion products
have been analysed by agarose gel electrophorese. (see figure below).
1
2
10,000
8,000
6,000
5,000
4,000
3,000
2,500
–2,000
- 1,500
-1,000
-750
- 500
-250
1. Digestion products
2. Size marker base pairs
Which restrcition enzyme(s) have been used for the digestion? (Indicate the right answer)
А- ЕсoRI.
B- EcoRI/BamHI.
C- EcoRI/Smal.
D- BamHI.
E- Smal.
| |
The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 109 In your answers, show how you came up to each result?
How many full double-helical turns does this DNA contain?
Question 1.
Enzymes, proteins and deoxyribonucleic acid (DNA)
macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA
repreduction.
are important biological
a) Compare the process of protein synthesis between eukaryote mRNA and viral RNA
b) With the aid of a diagram, draw an adapter molecule that recognizes the codons of mRNA
and explain its functions in DNA translation.
Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 17 - Prob. 1QCh. 17 - Prob. 2QCh. 17 - Prob. 3QCh. 17 - Prob. 4QCh. 17 - Prob. 5QCh. 17 - Prob. 6QCh. 17 - Prob. 7QCh. 17 - Prob. 8QCh. 17 - Prob. 1RQCh. 17 - Prob. 2RQ
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