Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 14RQ
Summary Introduction

To review:

Definition of the following terminologies:

1. Cistron

2. Operon

3.miRNA (micro ribonucleic Acid)

4.siRNA (small interfering RNA)

5.snoRNA (small nucleolar RNA)

Introduction:

The DNA (deoxyribonucleic acid) serves as the primary genetic constituent in most of the organisms. Certain genetic elements such as cistron and operons may be present in the DNA. Moreover, the DNA may code for noncoding RNA molecules such as miRNA, siRNA, and snoRNA.

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Question 2: Part a: Complete the table describing different components of intron removal from mRNA. Nu:, X and Y refer to B-type chemistry shown on the previous page. (YELLOW table shown) Part b: Complete the table describing different components of group I self-splicing intron removal from 26S rRNA in Tetrahymena. (BLUE table shown) Part c: Draw the intron with an all atom structure for Branchpoint A after intron removal from mRNA Part d: Draw the Group I self-splicing intron with an all atom structure for the Guanosine cofactor after intron removal from 26S rRNA in Tetrahymena.
The eukaryotic mRNA is protected from degradation by a 3′ _____________.
Suppose the codon sequence GUGCAAUUCGAGGCC has a single base pair mutation to GUGCAAUUCAAGGCC. If the old protein sequence was Val-Gln-Phe-Glu-Ala, what will be the new sequence encoded by the mutant gene? ____________________________.

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Biochemistry: The Molecular Basis of Life

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