Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 16RQ
Summary Introduction
To review:
The three biological properties that are facilitated by supercoiling.
Introduction:
Supercoiling of DNA (deoxyribonucleic acid) means the winding of DNA strand tighten enough to make it more compact. When the double helix of DNA further coils around its axis and crosses itsel, ft is calledsuper helix. Supercoiled DNA is mainly present in prokaryotes.
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Enzymes isolated from bacteria that cut DNA at specificsequences are called ____________________.
Multisubunit proteins in which some or all of the subunitsare identical are referred to as _______________________.
A posttranslational modification that directs a polypeptide toa specific site is called a _____________________modification.
Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
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- Question # 26: You are working with a genetic construct of your favorite protein, PTP1B, in the lab. You just performed a protein purification and are about to run an SDS-PAGE gel. You temporarily forgot PTP1B's molecular weight, but you know it is 300 residues long. Where on the gel would you expect to see a band for PTP18? Assume that the purification was successful, and that we are referring to the lane in the gel corresponding to the final, purified protein sample A) Near the ladder marker for 33 Da B) Near the ladder marker for 300 Da C) None of the other answers is correct D) Near the ladder marker for 300 kDa O Near the ladder marker for 33 kDa IDA= loo0,kba X ko Question #27: Which of the following methods would be most appropriate to determine high-resolution, atom-level structural information about a relatively small, difficult-to-crystallize protein with interesting dynamics? ANMR spectroscopy X-ray crystallography Cryo-electron microscopy PCD spectroscopy a SDS-PAGEarrow_forwardA base change that has no discernible effect is called a____________________ mutation.arrow_forwardQuestion 45 When TRNAS and rRNAs have bases that H-bonds with other bases far apart from each other, the RNA molecules assume its secondary and tertiary structure. A) True B) Falsearrow_forward
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- Question 6 The helical turns of the DNA does not only provide spaces for binding with regulatory proteins but they cause the molecule to resemble a spiral staircase. A) True B) Falsearrow_forwardQuestion 18 There is energy requirement for every amino acid added in a growing polypeptide chain. A) True B) Falsearrow_forwardQUESTION NO. 1 Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. The study of such patients has enhanced our knowledge of DNA repair because XP is caused by defective DNA repair nucleotide excision repair. (A variant, XP-V, is deficient in postreplication repair.) In nucleotide excision repair A. removal of the damaged bases occurs on only one strand of the DNA. B. only thymine dimers generated by UV light can be removed . C. the excision nuclease is an exonuclease. D. a single multifunctional enzyme carries out the repair process. E. only the damaged nucleotides are removed. QUESTION NO.2 Homologous recombination: A. occurs only between two segments from the same DNA molecule. B. requires that a specific DNA sequence be present. C. requires one of the duplexes undergoing recombination be nicked in both strands. D. involves a…arrow_forward
- The purpose of mitosis in human cells is for the production of two diploid, _________________ cells.arrow_forwardQuestion 1 In E. coli, the leading strand is synthesized discontinuously while the lagging strand is synthesized in one piece. A) True B) Falsearrow_forwardThe eukaryotic mRNA is protected from degradation by a 3′ _____________.arrow_forward
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