QUESTION 2 2.1 Recombinant plasmids are often used to produce therapeutic proteins. Elaborate on the steps you would follow to generate a recombinant plasmid that can be used in future to express a therapeutic protein of your choice. In your answer, include the important reagents used and their functions. 2.2 Thoroughly explain at least three different techniques you would use to confirm successful recombination in 2.1. In your answer, outline the principle behind each technique. 2.3 With supporting points, outline the Polymerase chain reaction (PCR) technique you would choose to amplify a desired gene from a Retrovirus.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
QUESTION 2
2.1 Recombinant plasmids are often used to produce therapeutic proteins. Elaborate on the
steps you would follow to generate a recombinant plasmid that can be used in future to
express a therapeutic protein of your choice. In your answer, include the important reagents
used and their functions.
2.2 Thoroughly explain at least three different techniques you would use to confirm successful
recombination in 2.1. In your answer, outline the principle behind each technique.
2.3 With supporting points, outline the Polymerase chain reaction (PCR) technique you would
choose to amplify a desired gene from a Retrovirus.
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