Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 13, Problem 6P
A diploid strain of yeast was made by mating a haploid strain with a genotype w-, x-, y-, and z- with a haploid strain of opposite mating type that is wild type for these four genes. The diploid strain was phenotypically wild type. Four different X-ray-induced diploid mutants with the following
Assume a single new mutation is present in each strain.
Strain 1 w- x+ y- z+
Strain 2 w+ x- y- z-
Strain 3 w- x+ y- z-
Strain 4 w- x+ y+ z+
When these mutant diploid strains of yeast go through meiosis, each ascus is found to contain only two viable haploid spores.
| a. | What kind of mutations were induced by X-rays to make the listed diploid strains? |
| b. | Why did two spores in each ascus die? |
| c. | Are any of the genes w, x, y, or z located on the same chromosome? |
| d. | Give the order of the genes that are found on the same chromosome. |
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Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction).
Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium alone, however, they can grow on medium supplemented with adenine.
All three yeast strains are homozygous for the underlying alleles.
When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium alone and require adenine supplementation;
when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium alone and do not require adenine.
A. What conclusions can you make about the alleles of mutant strains 1, 2, and 3 and their relationships with each other?
B. What phenomenon is occurring in the cross between mutant strains 1 and 3?
After crossing the F1 generation of the cross between mutant strains 1…
Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction).
Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium alone, however, they can grow on medium supplemented with adenine.
All three yeast strains are homozygous for the underlying alleles.
When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium alone and require adenine supplementation;
when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium alone and do not require adenine.
A. What conclusions can you make about the alleles of mutant strains 1, 2, and 3 and their relationships with each other?
B. What phenomenon is occurring in the cross between mutant strains 1 and 3?
A yeast geneticist irradiates haploid cells of a strain thatis an adenine-requiring auxotrophic mutant, caused bymutation of the gene ade1. Millions of the irradiatedcells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies.These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic,25% adenine-requiring auxotrophsa. Explain the difference between these two types ofresults.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predictfrom crossing a prototroph of type 2 by the original ade1auxotroph?
Chapter 13 Solutions
Genetics: From Genes to Genomes
Ch. 13 - For each of the terms in the left column, choose...Ch. 13 - Prob. 2PCh. 13 - For each of the following types of chromosomal...Ch. 13 - For the following types of chromosomal...Ch. 13 - One of the X chromosomes in a particular...Ch. 13 - A diploid strain of yeast was made by mating a...Ch. 13 - The two graphs that follow represent genomic...Ch. 13 - A series of chromosomal mutations in Drosophila...Ch. 13 - Indicate which of the four major classes of...Ch. 13 - The recessive, X-linked z1 mutation of the...
Ch. 13 - Genes a and b are 21 m.u. apart when mapped in...Ch. 13 - In the following group of figures, the pink lines...Ch. 13 - Three strains of Drosophila Bravo, X-ray, and...Ch. 13 - Two yeast strains were mated and sporulated...Ch. 13 - Suppose a haploid yeast strain carrying two...Ch. 13 - In the mating between two haploid yeast strains...Ch. 13 - During ascus formation in Neurospora, any...Ch. 13 - In the following figure, black and pink lines...Ch. 13 - In Drosophila, the gene for cinnabar eye color is...Ch. 13 - Semisterility in corn, as seen by unfilled ears...Ch. 13 - A promising biological method for insect control...Ch. 13 - Prob. 23PCh. 13 - a. Among the progeny of a self-fertilized...Ch. 13 - Duchenne muscular dystrophy DMD is caused by a...Ch. 13 - WHIM syndrome is a disease of the immune system...Ch. 13 - Explain how transposable elements can cause the...Ch. 13 - The Drosophila genome normally harbors about 40 P...Ch. 13 - Drosophila P elements were discovered because of a...Ch. 13 - Flies homozygous for mutant alleles of a...Ch. 13 - Fred and Mary have a child named Bob. The genomic...Ch. 13 - Uniparental disomy is a rare phenomenon in which...Ch. 13 - Among adults with Turner syndrome, it has been...Ch. 13 - In Neurospora, his2 mutants require the amino acid...Ch. 13 - Human geneticists interested in the effects of...Ch. 13 - The incidence of Down syndrome will be very high...Ch. 13 - The Drosophila chromosome 4 is extremely small;...Ch. 13 - Down syndrome is usually caused by having a...Ch. 13 - Common red clover, Trifolium pratense, is a...Ch. 13 - The numbers of chromosomes in the somatic cells of...Ch. 13 - Prob. 41PCh. 13 - Somatic cells in organisms of a particular diploid...Ch. 13 - An allotetraploid species has a genome composed of...Ch. 13 - Prob. 44PCh. 13 - Chromosomes normally associate during meiosis I as...Ch. 13 - Using whole-genome sequencing, how could you...Ch. 13 - Prob. 47PCh. 13 - Prob. 48PCh. 13 - Seedless watermelons that you find in the...Ch. 13 - The names of hybrid animals are usually themselves...Ch. 13 - While most animals cannot tolerate polyploidy,...Ch. 13 - What characteristic property of translocations...Ch. 13 - Prob. 53PCh. 13 - In the accompanying figure, the top and bottom...Ch. 13 - Prob. 55PCh. 13 - The accompanying figure shows idiograms of human...Ch. 13 - Prob. 57P
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