Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 13, Problem 43P
An allotetraploid species has a genome composed of two ancestral genomes, A and B, each of which have a basic chromosome number (x) of seven. In this species, the two copies of each chromosome of each ancestral genome pair only with each other during meiosis. Resistance to a pathogen that attacks the foliage of the plant is controlled by a dominant allele at the F locus. The recessive alleles Fa and Fb confer sensitivity to the pathogen, but the dominant resistance alleles present in the two genomes have slightly different effects. Plants with at least one FA allele are resistant to races 1 and 2 of the pathogen regardless of the genotype in the B genome, and plants with at least one FB allele are resistant to races 1 and 3 of the pathogen regardless of the genotype in the A genome. What proportion of the self-progeny of an FA Fa FB Fb plant will be resistant to all three races of the pathogen?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
Chapter 13 Solutions
Genetics: From Genes to Genomes
Ch. 13 - For each of the terms in the left column, choose...Ch. 13 - Prob. 2PCh. 13 - For each of the following types of chromosomal...Ch. 13 - For the following types of chromosomal...Ch. 13 - One of the X chromosomes in a particular...Ch. 13 - A diploid strain of yeast was made by mating a...Ch. 13 - The two graphs that follow represent genomic...Ch. 13 - A series of chromosomal mutations in Drosophila...Ch. 13 - Indicate which of the four major classes of...Ch. 13 - The recessive, X-linked z1 mutation of the...
Ch. 13 - Genes a and b are 21 m.u. apart when mapped in...Ch. 13 - In the following group of figures, the pink lines...Ch. 13 - Three strains of Drosophila Bravo, X-ray, and...Ch. 13 - Two yeast strains were mated and sporulated...Ch. 13 - Suppose a haploid yeast strain carrying two...Ch. 13 - In the mating between two haploid yeast strains...Ch. 13 - During ascus formation in Neurospora, any...Ch. 13 - In the following figure, black and pink lines...Ch. 13 - In Drosophila, the gene for cinnabar eye color is...Ch. 13 - Semisterility in corn, as seen by unfilled ears...Ch. 13 - A promising biological method for insect control...Ch. 13 - Prob. 23PCh. 13 - a. Among the progeny of a self-fertilized...Ch. 13 - Duchenne muscular dystrophy DMD is caused by a...Ch. 13 - WHIM syndrome is a disease of the immune system...Ch. 13 - Explain how transposable elements can cause the...Ch. 13 - The Drosophila genome normally harbors about 40 P...Ch. 13 - Drosophila P elements were discovered because of a...Ch. 13 - Flies homozygous for mutant alleles of a...Ch. 13 - Fred and Mary have a child named Bob. The genomic...Ch. 13 - Uniparental disomy is a rare phenomenon in which...Ch. 13 - Among adults with Turner syndrome, it has been...Ch. 13 - In Neurospora, his2 mutants require the amino acid...Ch. 13 - Human geneticists interested in the effects of...Ch. 13 - The incidence of Down syndrome will be very high...Ch. 13 - The Drosophila chromosome 4 is extremely small;...Ch. 13 - Down syndrome is usually caused by having a...Ch. 13 - Common red clover, Trifolium pratense, is a...Ch. 13 - The numbers of chromosomes in the somatic cells of...Ch. 13 - Prob. 41PCh. 13 - Somatic cells in organisms of a particular diploid...Ch. 13 - An allotetraploid species has a genome composed of...Ch. 13 - Prob. 44PCh. 13 - Chromosomes normally associate during meiosis I as...Ch. 13 - Using whole-genome sequencing, how could you...Ch. 13 - Prob. 47PCh. 13 - Prob. 48PCh. 13 - Seedless watermelons that you find in the...Ch. 13 - The names of hybrid animals are usually themselves...Ch. 13 - While most animals cannot tolerate polyploidy,...Ch. 13 - What characteristic property of translocations...Ch. 13 - Prob. 53PCh. 13 - In the accompanying figure, the top and bottom...Ch. 13 - Prob. 55PCh. 13 - The accompanying figure shows idiograms of human...Ch. 13 - Prob. 57P
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- 1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forward1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Mechanisms of Genetic Change or Evolution; Author: Scientist Cindy;https://www.youtube.com/watch?v=5FE8WvGzS4Q;License: Standard Youtube License