30- 20- hisEt pheA+ 10 - O- 10 20 30 40 50 Duration of conjugation (minutes) % of F- reciplent cells that have recelved the gene during conjugation
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The conjugation was interrupted at different times, and the percentage of recombinants for each gene was determined by streaking on media that lacked either histidine or phenylalanine. The following results were obtained:
A. Determine the map distance (in minutes) between these two
genes.
B. In a previous experiment, it was found that hisE is 4 minutes
away from pabB and that PheA is 17 minutes from pabB. Draw
a genetic map showing the locations of all three genes.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
