Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 12, Problem 17P
Summary Introduction

a.

To determine:

The reason duuue to which X chromosome will convert into Barr body.

Introduction:

The process of DNA replication is the process by which the genetic material of the organism copies itself to be distributed among the daughter cells during cell division. The DNA replication takes place before the cell division and prepares the cell for mitosis and meiosis. This process is followed by transcription and translation.

Summary Introduction

b.

To determine:

The explanation for interference in the expression of Xist present on the same chromosome as Tsix.

Introduction:

The chromosome from chromatids is formed through the process of supercoiling. The chromatin fibers are condensed into thick structures called the chromosomes. This chromosome in bacteria is circular, whereas in eukaryotes it is linear.

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Xist gene is located in a 450 kb region of DNA called the X inactivation center (XIC) and is transcribed only from the future inactive X chromosome. The Xist noncoding RNA (ncRNA) triggers X inactivation by coating the X chromosome that produces it and recruiting enzymes that allow Barr body formation. How one X chromosome is chosen to express Xist is unknown. Active X Inactive X Xist XIC • Histone modifying proteins ~ Xist ncRNA Which of the following scenarios will cause the maternal X chromosome to condense into a Barr body? Select all that apply. O Histone deacetylation of the promoter region of paternal Xist gene DNA methylation of the promoter region of patemal Xist gene O Gain-of-function mutation in the repressor of Xist transcription O Transcriptional repression of the maternal Xist gene O Deletion of Xist promoter on maternal X chromosome Deletion of XIC on maternal X chromosome
Shown below is the genomic structure of the human B-globin gene. The numbers within the boxes indicate the length in nucleotides of each region. Question 6: How many amino acids are present in the wild-type human B-globin protein? = exons = introns Transcription termination site (also poly A site) Promoter Start of transcription 3' 5' ATG 50 TẠC TAA 126 132 |ATT 90 130 222 850 3 5' Start codon Stop codon А. 438 В. 146 C. 620 D. 206 © 2013 John Wiley & Sons, Inc. All rights reserved.
GR and PPAR are transcription factors that bind to GRE and PPARE sequences respectively  and activate transcription of genes. A reporter cell line is created in which the the green fluoresecent protein (GFP) is controlled by a GRE sequence and the pink fluorescent protein mCherry is under control of a PPARE sequence. If the gene for GR is introduced into the reporter cell line, the cells produce a green color. Chimeric proteins are created in which the DNA Binding Domains (DBD) and Activation Domains (AD) of the transcription factors are introduced into various cell lines. Match the following cell-types with the fluorescent color(s) you would expect the cells to produce.

Chapter 12 Solutions

Genetics: From Genes to Genomes

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