Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 12, Problem 14P
Recently, scientists constructed a transgene that expresses a mutant form of Drosophila histone H3 in which lysine 27 in the histone tail was changed to methionine (H3K27M). Expression of the H3K27M transgene results in aberrant development of fruit flies because of inappropriate expression of many different genes. Explain this finding.
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In a mutant mouse, it was discovered that a gene "W" is moved from its original
chromosomal locus to the telomere region, where chromatin is packed into
heterochromatin. Given what we have learned about eukaryotic gene regulation, can
you speculate how the expression of gene W in the new mouse line would be
changed as compare to the normal mouse?
elevated in the mutant mouse than in the wild-type mouse.
cannot be determined.
O reduced in the mutant mouse than in the wild-type mouse.
change in the mutant mouse as compared to the wild-type mouse.
In a mutant mouse, it was discovered that a gene "W" is moved from its original chromosomal locus to the telomere region, where chromatin is packed into heterochromatin. Given what we have learned about eukaryotic gene regulation, can you speculate how the expression of gene W in the new mouse line would be changed as compare to the normal mouse?
Question 18 options:
elevated in the mutant mouse than in the wild-type mouse.
cannot be determined.
reduced in the mutant mouse than in the wild-type mouse.
no change in the mutant mouse as compared to the wild-type mouse.
Recently, scientists constructed a transgene that expresses a mutant form of Drosophila histone H3 inwhich lysine 27 in the histone tail was changed to methionine (H3K27M). Expression of the H3K27Mtransgene results in aberrant development of fruit fliesbecause of inappropriate expression of many differentgenes. Explain this finding.
Chapter 12 Solutions
Genetics: From Genes to Genomes
Ch. 12 - For each of the terms in the left column, choose...Ch. 12 - Many proteins other than histones are found...Ch. 12 - What difference exists between the compaction of...Ch. 12 - What is the role of the core histones in...Ch. 12 - a. About how many molecules of histone H2A would...Ch. 12 - The enzyme micrococcal nuclease can cleave...Ch. 12 - a. What letters are used to represent the short...Ch. 12 - About 2000 G bands are visible in a...Ch. 12 - Suppose you performed a fluorescence in situ...Ch. 12 - Which of the following would be suggested by a...
Ch. 12 - For each of the following pairs of chromatin...Ch. 12 - a. Drosophila b. Humans Give examples of...Ch. 12 - One histone modification that is seen consistently...Ch. 12 - Recently, scientists constructed a transgene that...Ch. 12 - Drosophila geneticists have isolated many...Ch. 12 - On the following figures, genes A and B are on the...Ch. 12 - Prob. 17PCh. 12 - The first page of this chapter displays photos of...Ch. 12 - The human genome contains about 3 billion base...Ch. 12 - The mitotic cell divisions in the early embryo of...Ch. 12 - In an experiment published in the journal Cell in...Ch. 12 - a. What DNA sequences are found at the telomeres...Ch. 12 - Prob. 23PCh. 12 - a. In a fluorescent in situ hybridization FISH...Ch. 12 - If you are comparing the two telomeres in each...Ch. 12 - a. What DNA sequences are commonly found at human...Ch. 12 - On the graphs presented in Problem 21, no data is...Ch. 12 - Prob. 29PCh. 12 - Prob. 30PCh. 12 - In the 1920s, Barbara McClintock, later a Nobel...Ch. 12 - Give at least one example of a chromosomal...Ch. 12 - Cornelia de Lange syndrome CdLS is a rare human...Ch. 12 - a. Give at least three examples of types of...Ch. 12 - A number of yeast-derived elements were added to...Ch. 12 - Prob. 36PCh. 12 - The completely synthetic yeast chromosome Syn III...
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- Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid +. To study these mutations, phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and the results are shown below. (Anterior is on the left; posterior is on the right.) Northern blot 1 2 - 3 4 In situ hybridization Wild type Lane 1. Wild type (bicoid*) Lane 2. bicoid-A Lane 3. bicoid-B Lane 4. bicoid-C bicoid-B bicoid-A bicoid-C Which mutation is likely to cause the embryo to develop two "anterior" ends? bicoid-B Obicoid-A bicoid-Carrow_forwardFlowering Locus C (FLC) is a gene that is responsible for flowering in certain plants. FLC is normally expressed in new seedlings, which prevents flowering. When a plant is exposed to cold temperatures, FLC expression decreases and the plant produces flowers. FLC is regulated through epigenetic modifications. Based on this information, you should expect which of the following in histone proteins near the FLC gene: 1.In new seedlings, histones will be acetylated; while in the cold, histone methylation occurs 2.In new seedlings, histones will be methylated; while in the cold, histone acetylation occurs 3.In new seedlings, histones will be methylated; while in the cold, histone deacetylation occursarrow_forwardLysine 4 of histone H3 (H3K4) is methylated in thenucleosomes of many transcriptionally active genes.Suppose you want to determine all the places in thehuman genome where nucleosomes contain methylated H3K4.a. Starting with an antibody that specifically bindsonly to the tails of histone H3s that have K4 methylation, what kind of experiment would you perform? Outline the major steps of this experiment.b. Do you think that you would get the same results ifyour starting material was skin cells in one experiment and blood precursor cells in a second experiment? Explain.c. Describe a follow-up experiment that could determine if your data from part (a) are consistent withthe idea that H3K4 methylation marks appear onlyat transcriptionally active genesarrow_forward
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