Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 9.1, Problem 3TQ
Summary Introduction
To review:
The protection of lysogen from generalized transduction carried out by a similar phage.
Introduction:
Transferring of DNA (deoxyribonucleic acid) of one cell to another cell is an evident process in the field of
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phage can be "induced" as a result of DNA damage. Consequences of
induction include.
Temperate phage, e.g lambda phage; lytic cycle and transduction
Lytic T4 phage, assembly, conjugation
O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation
Temperate phage, e.g. lambada phage, prophage formation, transformation
O T4 phage, lytic cycle, specialized trasnduction
Which of the following statements describes the lysogenic cycle of lambda (A) phage?
After infection, the viral genes immediately turn the host cell into a lambda-producing factory, and the host
cell then lyses.
Most of the prophage genes are activated by the product of a particular prophage gene.
The phage DNA is incorporated into the bacteria DNA
The phage DNA is copied and exits the cell as a phage.
The phage DNA get destructed by the host bacteria DNA
What is the difference between the lytic and lysogenic cycle of bacteriophages?
In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change
The host cell is not killed in the lysogenic cycle
The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm
In the lysogenic cycle, penetration is achieved through phagocytosis
Chapter 9 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 9.1 - Prob. 1TQCh. 9.1 - Prob. 2TQCh. 9.1 - Prob. 3TQCh. 9.1 - Prob. 4TQCh. 9.2 - Prob. 1TQCh. 9.3 - Prob. 1TQCh. 9.4 - Prob. 1TQCh. 9.4 - Prob. 2TQCh. 9.5 - Prob. 1TQCh. 9.6 - Prob. 1TQ
Ch. 9 - Prob. 1RQCh. 9 - Prob. 2RQCh. 9 - Prob. 3RQCh. 9 - Prob. 4RQCh. 9 - Prob. 5RQCh. 9 - Prob. 6RQCh. 9 - Prob. 7RQCh. 9 - Prob. 8RQCh. 9 - Prob. 9RQCh. 9 - Prob. 10RQCh. 9 - Prob. 11RQCh. 9 - Prob. 12RQCh. 9 - Prob. 13RQCh. 9 - Prob. 14RQCh. 9 - Prob. 15RQCh. 9 - Prob. 16RQCh. 9 - Prob. 1TQCh. 9 - Prob. 2TQCh. 9 - Prob. 3TQCh. 9 - Prob. 4TQCh. 9 - Prob. 5TQCh. 9 - Prob. 6TQ
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- You are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forwardWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forwardThis gene is responsible for making the protein that catalyzes the recombination of the phage genome into the host bacterial chromosome is: excise integrase attP attBarrow_forward
- Host DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?arrow_forwardDescribe the reason behind the occurrence of phage growth and release by the mating with the non-lysogenic recipient, but not with the infection by a lysogenic recipient.arrow_forwardWhy does phage T4 need a lysozyme-like protein in order toinfect its host, and what part of T4 enters the host cytoplasm?arrow_forward
- A phage that reproduces slowly, produces relatively few phage particles, and only undergoes the lytic life cycle most likely will produce a plaque that is: Smaller and clear Smaller and turbid/hazy Larger and turbid/hazy Larger and cleararrow_forwardWhat advantages might a phage gain by being capable of lysogeny?arrow_forwardClassify each statement as true of generalized transduction or specialized transduction. Generalized transduction Specialized transduction Answer Bank Genes transfer from random sites in the bacterial chromosome. This utilizes lysogenic phages. Genes that transfer depend on the prophage insertion site. This utilizes lytic phages. Phage inserts itself at a specific site in the bacterial chromosome. This requires accidental packaging of bacterial DNA in viral capsids.arrow_forward
- Lambda phage çan facilitate bacterial chemosomal DNA transfer through the process of specialized transduction / generalized transduction (copy and paste the correct answer) A by transferring specific bacterial DNA / bacterial DNA at random (copy and paste the correct answer) A from a donor cell / recipient cell (copy and paste the correct answer) A to a donor cell / recipient cell (copy and paste the correct answer).arrow_forwardMost phages that use DNA to propagate work by hijacking host bacterial machinery including RNA Polymerase. Scientists found that treatment of jumbo bacteriophage PhiKZ with Rifampicin did not stop the phage from propagating. What is allowing for the survival of this phage?arrow_forwardPut in the correct order the following steps of the lytic cycle in phage replication: 1. Phage components are assembled into mature virions 2. Phage attach to a host cell 3. The bacterial cell lyses and many new infectious virions are released 4. Phage DNA is injected into the bacterial cell 5. Copies of phage genome and phage proteins are made, the host DNA is degradedarrow_forward
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