The following: 1) The reason for using Agrobacterium tumefaciens Ti plasmid as a vector for breeding new plants. 2) The possibility of a genetically altered plant to develop a tumor in the given condition. Introduction: Agrobacterium tumefaciens is a Gram-negative rod-shaped bacterium. This bacterium travels toward the wounded plants that release certain types of metabolic compounds. It has a large plasmid called Tumor inducing plasmid (Ti plasmid). This bacteria is used as a vector for the introduction of genes in the plants and is termed as natureâ€™s genetic engineer.

Question

Want to see the full answer?

Answer 1

Question

Chapter 9, Problem 4TQ

Summary Introduction

To review:

The following:

1) The reason for using Agrobacterium tumefaciens Ti plasmid as a vector for breeding new plants.

2) The possibility of a genetically altered plant to develop a tumor in the given condition.

Introduction:

Agrobacterium tumefaciens is a Gram-negative rod-shaped bacterium. This bacterium travels toward the wounded plants that release certain types of metabolic compounds. It has a large plasmid called Tumor inducing plasmid (Ti plasmid). This bacteria is used as a vector for the introduction of genes in the plants and is termed as natureâ€™s genetic engineer.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

What is the role of the Ti plasmid in plant tumor formation?

You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a test group of strawberry plants. Select the events you would use to genetically engineer these plants. Insert the ice gene into the T-DNA region of a Ti plasmid; transform Agrobacterium tumefaciens with this plasmid; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. Insert the ice gene into the T-DNA region of the Agrobacterium tumefaciens chromosome; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. O Insert the ice gene into a Ti transposon; Introduce the transposon into Agrobacterium tumefaciens Ti plasmid: Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens: Select plant cells that have taken up the ice gene. Insert the ice gene into a Ti bacteriophage; transduce Agrobacterium tumefaciens with this…

Scientists modified the tumor-inducing (Ti) plasmid, a naturally occurring plant plasmid found in Agrobacterium tumefaciens, to create a tool used to introduce any gene of interest into plant cells. They created a binary system because a single Ti plasmid is too large to be easily manipulated. One part of the system is a disarmed plasmid, and the second part is a transformation vector. The following sentences describe the function of key DNA elements in the system. Virulence region Genes for conjugative transfer Disarmed Ti plasmid (T-region removed) ori Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer virulence Kan (bacterial selectable marker) Amp selectable marker Plant selectable marker (e.g., herbicide resistance) Constitutive promoter T-DNA left border Place the terms in the appropriate blanks to complete the sentences. Not all terms will be used. 3' transcriptional terminator MCS (inserted gene of interest) T-region Transformation…