Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 9.1, Problem 4TQ
Summary Introduction
To review:
The phage DNA containing the restriction site evades the restriction-modification screening system of its host.
Introduction:
A system present in cells to distinguish between self DNA (deoxyribonucleic acid) and foreign DNA is the restriction-modification system. It involves cleaving of foreign DNA at specific restriction sites and methylation of self DNA. The bacteria produce certain specialized enzymes called restriction endonucleases to identify as well as cleave the DNA at specific sites.
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Why is it important to create a restriction map/ vector map?
The ability of F+ cells, or Hfr cells, to transfer plasmid DNA to an F- cell is properly called:
transversion
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Why do bacteria make restriction endonucleases?
Chapter 9 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 9.1 - Prob. 1TQCh. 9.1 - Prob. 2TQCh. 9.1 - Prob. 3TQCh. 9.1 - Prob. 4TQCh. 9.2 - Prob. 1TQCh. 9.3 - Prob. 1TQCh. 9.4 - Prob. 1TQCh. 9.4 - Prob. 2TQCh. 9.5 - Prob. 1TQCh. 9.6 - Prob. 1TQ
Ch. 9 - Prob. 1RQCh. 9 - Prob. 2RQCh. 9 - Prob. 3RQCh. 9 - Prob. 4RQCh. 9 - Prob. 5RQCh. 9 - Prob. 6RQCh. 9 - Prob. 7RQCh. 9 - Prob. 8RQCh. 9 - Prob. 9RQCh. 9 - Prob. 10RQCh. 9 - Prob. 11RQCh. 9 - Prob. 12RQCh. 9 - Prob. 13RQCh. 9 - Prob. 14RQCh. 9 - Prob. 15RQCh. 9 - Prob. 16RQCh. 9 - Prob. 1TQCh. 9 - Prob. 2TQCh. 9 - Prob. 3TQCh. 9 - Prob. 4TQCh. 9 - Prob. 5TQCh. 9 - Prob. 6TQ
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- What do you mean by vector plasmid?arrow_forwardLane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindlII+ Pstl. label on the plasmid. i) The location of the Pstl site ii) The sizes of the new fragments made by this Pstl cutarrow_forwardWhat functions must a plasmid encode to be self-transmissible?arrow_forward
- What uses do Type 1 restriction enzymes have if they seem to be so inaccurate?arrow_forwardE. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interferencearrow_forwardWhat is the function of a restriction enzyme?arrow_forward
- a) If lacZ was successfully inserted into the plasmid and E. coli successfully transformed, then the lacZ gene will be expressed which will breakdown X-gal producing a blue-colored colony. b) If the gene of interest was successfully inserted into the plasmid and E. coli successfully transformed, then the LacZ gene will not be expressed so X-gal will be broken down leading to a blue-colored colony. c) If the gene of interest was successfully inserted into the plasmid and E. coli successfully transformed, then the LacZ gene will not be expressed so X-gal will not be broken down leading to a white-colored colony. d) If lacZ was successfully inserted into the plasmid and E. coli successfully transformed, then the lacZ gene will be expressed which will breakdown X-gal producing a white-colored colony.arrow_forwardWhat is pGLO plasmid? How has it been engineered so that the cells that carry it can be detected? Attach a map of it.?arrow_forwardThe linear dsDNA genome of λ binds on the LamB receptor of E. Coli and conducts a normal lysogenic cycle. Exposure to stress will cause the excision of λ prophage from the E. Coli genome. The excised λ genome is then replicated, packaged, and released from the cell as mature λ phage particles and ready to infect other bacterial cells. Among λ phage particles,the transducing phage mediates a specific type of recombination. Understand this scenario and answer the following questions. 1. What are the basic requirements for the insertion of λ into the E. Coli genome? 2. What special features are found in the λ insertion site? 3. What type of recombination occurs with λ insertion in the E. Coli genome? 4. How you will differentiate λ transducing phage from normal λ phage? 5. What exclusive mechanism λ phage utilizes for recombination?arrow_forward
- What are Bacterial Plasmid Vectors ?arrow_forwardBacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceA+ thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace str: gene involved in streptomycin resistance, aceA gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 Determine the order of the genes and draw a genetic map showing this orderarrow_forwardIn a study, bacterial culture of E. coli was infected with bacteriophage. How- ever, these cultures were protected from phage infection. Interestingly, it was found that the resistance is due to endonudeases present in E. coli, which deaved the phage DNA. How is the E. coli genomic DNA protected from the action of these endonuclease enzymes?arrow_forward
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