Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 9, Problem 5TQ
Summary Introduction
To review:
The failure in identifying the source species from which the genetic material has been horizontally transferred to another species.
Introduction:
Transformation is a process by which a living cell uptakes the DNA (deoxyribonucleic acid) from a dead or lysed cell. The movement of genes within the same species is called horizontal gene transfer. Transformation is one of the three forms of horizontal gene transfer. It takes place in all forms of organisms.
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We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media:
A) Donor and recipient strain mixed together (control - no treatment).
B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells).
The results:
Tubes A, B, and D: HGT was observed.
Tube C: HGT was NOT observed.
Based on this, which type of HGT was occurring?
Conjugation,…
All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1 phage except:
A) it served as an example of an inflexible test
B) it involved looking for T1-resistant bacteria as an end measure
C) it showed that numbers of mutant organisms observed after T 1 phage were added to the culture tended to vary from experiment to experiment
D) it rejected the possibility of adaptive mutations
E) it supported the concept of spontaneous mutation rates
17) What gives Aspergillus sydowii colonies their hairy appearance?
a) Hyphae
() b) Stipes
c) Growing media
) d) Ascocarpoda
Jack has designed one primer which is complementary to the DNA of E. coli. He
then used that primer for a PCR reaction using DNA isolated from a mixture of
bacteria. He got no PC reaction product and hence concluded that there is
absolutely no E. coli in the bacteria mixture. His conclusion is ....
() True
) False
Chapter 9 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 9.1 - Prob. 1TQCh. 9.1 - Prob. 2TQCh. 9.1 - Prob. 3TQCh. 9.1 - Prob. 4TQCh. 9.2 - Prob. 1TQCh. 9.3 - Prob. 1TQCh. 9.4 - Prob. 1TQCh. 9.4 - Prob. 2TQCh. 9.5 - Prob. 1TQCh. 9.6 - Prob. 1TQ
Ch. 9 - Prob. 1RQCh. 9 - Prob. 2RQCh. 9 - Prob. 3RQCh. 9 - Prob. 4RQCh. 9 - Prob. 5RQCh. 9 - Prob. 6RQCh. 9 - Prob. 7RQCh. 9 - Prob. 8RQCh. 9 - Prob. 9RQCh. 9 - Prob. 10RQCh. 9 - Prob. 11RQCh. 9 - Prob. 12RQCh. 9 - Prob. 13RQCh. 9 - Prob. 14RQCh. 9 - Prob. 15RQCh. 9 - Prob. 16RQCh. 9 - Prob. 1TQCh. 9 - Prob. 2TQCh. 9 - Prob. 3TQCh. 9 - Prob. 4TQCh. 9 - Prob. 5TQCh. 9 - Prob. 6TQ
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