Why is it important to include a selective marker gene on a plasmid? O a) So that you can select cells containing the plasmid after transformation. b) So that you can select where the plasmid inserts into the genome. c) So that you can select the recombinant gene you want to add to the plasmid. d) So that you can select when the recombinant gene on your plasmid is expressed.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Bacteria are common source of plasmids. For the sole purpose of Cloning experiments, new plasmids with specific features are constructed. Such artificial plasmids are superior and efficient. Example - pBR 322, pUC 118 and 119.
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