Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 8.6, Problem 1TQ
Summary Introduction

To analyze:

The size of a protein encoded by an ORF 1,200bp (base pairs) in length.

Introduction:

An ORF (operon reading frame) is a continuous stretch of mRNA (messenger ribonucleic acid) that starts from the initiator codon, most commonly AUG and ends at a stop codon (UAA, UAG or UGA). It is a part of the translation unit. Various computer programs are available to find ORFs that use universal genetic code to deduce a protein sequence.

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An ORF 1,200 bp in length can encode a protein of what size?
Estimate the time needed for E. coli RNA polymerase at 37 °C to transcribe the entire gene for a 50-kilodalton protein. What assumption or assumptions must be made for this estimate to be accurate?
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