Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 8.1, Problem 3TQ
Summary Introduction
To review:
The significance of the presence of multiple promoters in a gene which are specific for different sigma factors.
Introduction:
The specific DNA (deoxyribonucleic acid) sequence to which the RNA (ribonucleic acid) polymerase binds is called the promoter site. The sigma factor helps the polymerase to find the promoter region on the gene. Transcription is a process where the information on the DNA strand is copied on the RNA strand.
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Which promoter initiates which life cycle (lysogenic & Lytic)? Mention both types of promoters and their characteristics.
In bacteria, which sequences in the promoter does sigma protein recognize to
establish a constant position with respect to the start of transcription?
-30 and -60
-10 and -60
-10 and -35
-35 and -60
Answer these questions concerning promoters.
a) What role do promoters play in transcription?
b) What is the common structure of bacterial promoter with respect to consensus sequences?
c) Eukaryotic promoters are more variable than bacterial promoters. Why?
d) What is the meaning of the term alternative promoter? How does the use of alternative promoters affect transcription?
Chapter 8 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 8.1 - Prob. 1TQCh. 8.1 - Prob. 2TQCh. 8.1 - Prob. 3TQCh. 8.3 - Prob. 1TQCh. 8.3 - Prob. 2TQCh. 8.3 - Prob. 3TQCh. 8.3 - Prob. 4TQCh. 8.3 - Prob. 5TQCh. 8.3 - Prob. 6TQCh. 8.3 - Prob. 7TQ
Ch. 8.3 - Prob. 8TQCh. 8.6 - Prob. 1TQCh. 8 - Prob. 1RQCh. 8 - Prob. 2RQCh. 8 - Prob. 3RQCh. 8 - Prob. 4RQCh. 8 - Prob. 5RQCh. 8 - Prob. 6RQCh. 8 - Prob. 7RQCh. 8 - Prob. 8RQCh. 8 - Prob. 9RQCh. 8 - Prob. 10RQCh. 8 - Prob. 11RQCh. 8 - Prob. 12RQCh. 8 - Prob. 13RQCh. 8 - Prob. 14RQCh. 8 - Prob. 15RQCh. 8 - Prob. 16RQCh. 8 - Prob. 17RQCh. 8 - Prob. 1TQCh. 8 - Prob. 2TQCh. 8 - Prob. 3TQ
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- In bacteria, genes that are often used together are controlled by a single promoter. Explain why this is the case.arrow_forwardWhich of the following is a property or characteristic of eukaryotic promoters? They contain the -10 and-35 consensus sequences where RNA polymerase holoenzyme, containing the sigma factor, bind to initiate transcription. | They contain the -10 and -35 consensus sequences that are the site where general transcription factors bind to help RNA polymerase holoenzyme initiate transcription. They contain a TATA box to which sigma factors bind to help RNA polymerase core enzyme initiate transcription. O I. They are usually located far away from the gene that is being transcribed. IV. OV. A, They are bound by general transcription factors, which help RNA polymerase I, II, or III, bind to the promoter and initiate transcription.arrow_forwardWhat are the role of transcription proteins? Explain briefly at your own wordsarrow_forward
- The sigma-70 promoter is very well-studied. The Sigma-54 promoter, on the other hand, hasn't had quite as much study devoted to it, but a consensus sequence for Sigma-54 promoters has been determined, and it looks like this: C GAAA FICGCACTECT УА C 5-26-25-24 -23 -22 -21 -20 -19 -18 -17 -16-15-14-13-12-11 -10 3 wablogo berkalay.edu (From Franke et. Al, 2011 BMC Genomics) If you were studying a gene with a promoter that exactly matched the consensus sequence, which base would, if mutated, probably make the largest difference in the ability of Sigma-54 to bind to a promoter? O Base position 26 Base position 10 Base position 12 Base position 16arrow_forwardWhat are the advantages of having multiple types of promoters and enhancers?arrow_forwardWhat are strong promoters?arrow_forward
- The sigma factor protein's role in transcription in E. coli includes which of the following? None of the answer options are correct. plays a role in transcription termination forms part of the core enzyme required for transcription initiation helps the siRNA to bind to the promoter All of the answer options are correct. contributes to the proof-reading activity of RNA polymerase And The role of tRNA is to serve as an intermediate in the decoding of genes. serve as general translational components of the ribosome. facilitate protein trafficking in protein secretion. facilitate splicing of pre-messenger RNAs. act as vehicles bringing amino acids to the site of protein synthesis. None of the answer choices are correct.arrow_forwardTFIIA Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a a) may stabilize binding between TFIID and the promoter b b) is the first factor to bind c) is important in transcription for RNA pol I d) none of the abovearrow_forwarda) what is a promoter and give the element and their functions of E.coli promoter b) what are eukaryotic transcription factor and list the class 2 general transcription factors and state their functionsarrow_forward
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