Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 8, Problem 1TQ
Summary Introduction

To review:

The mechanism that releases positive supercoils in the DNA (Deoxyribonucleic acid) which help DNA polymerase to move along the DNA template during the transcription process.

Introduction:

Transcription is the process in which the DNA sequences of a gene are copied to produce RNA (Ribonucleic acid). RNA polymerase acts as the main transcription enzyme. The process of transcription proceeds in three stages: initiation, elongation, and termination. Initiation is the beginning of transcription, elongation is the polymerization of RNA, and the last stage is termination in which the RNA molecule is released from the transcription complex.

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What is the difference between the leading strand and the lagging strand in DNA replication?     There are different DNA polymerases involved in elongation of the leading strand and the lagging strand.     The leading strand is synthesized continuously in the 5' → 3' direction, while the lagging strand is synthesized discontinuously in the 5' → 3' direction.     The leading strand requires an RNA primer, whereas the lagging strand does not.     The leading strand is synthesized in the 3' → 5' direction in a discontinuous fashion, while the lagging strand is synthesized in the 5' → 3' direction in a continuous fashion.
DNA polymerases are processive, which means that they remain tightly associated with the template strand while moving rapidly and adding nucleotides to the growing daughter stand. Which piece of the replication machinery accounts for this characteristic? Helicase Sliding Clamp Single Stranded Binding Protein Primase
Which of the followings statements are true about DNA polymerase? 1.) It can only go in one direction, meaning the lagging strand can't be synthesized continuously. 2.) It cannot start a DNA strand from scratch, so another enzyme is needed to create "primers" as a starting point. 3.) It cannot copy epigenetic marks (such as methyl groups) on its own; these must be "copied" onto the daughter DNA strand by other enzymes after DNA replication. 4.) All of the above
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