The study of changed gene expression due to mutation. Introduction: Translation is the process of synthesis of proteins from an mRNA (messenger ribonucleic acid) template. Information is present in the form of a sequence of nucleotide which is translated into the amino acid sequence of proteins using ribosomes as a catalyst. Translation initiates from the initiator codon, most commonly AUG codon that codes for methionine and terminates at the stop codon UAA, UGA, and UAG.

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Chapter 8.3, Problem 3TQ

Summary Introduction

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The study of changed gene expression due to mutation.

Introduction:

Translation is the process of synthesis of proteins from an mRNA (messenger ribonucleic acid) template. Information is present in the form of a sequence of nucleotide which is translated into the amino acid sequence of proteins using ribosomes as a catalyst. Translation initiates from the initiator codon, most commonly AUG codon that codes for methionine and terminates at the stop codon UAA, UGA, and UAG.

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The diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the mRNA leader region. Suppose a mutant bacteria has region 3 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Leader region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA AUG UGGUGG UUUUUU 1 2 3 (tryptophan codons) There is insufficient information to answer the question. Yes No O O

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