The overall gene expression in the cell when one sigma factor is artificially overexpressed and the effect of this overexpression on growth. Introduction: Transcription is the process of copying the genetic information from the template strand of the DNA (deoxyribonucleic acid) to RNA (ribonucleic acid). The enzyme complex involved in this process is called RNA polymerase. It consists of a protein that is involved in the polymerization of RNA (core enzyme) and a protein needed to initiate transcription (sigma factor). Sigma factor helps RNA polymerase to recognize the transcription start site or the promoter site.

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 8.1, Problem 2TQ

Summary Introduction

To review:

The overall gene expression in the cell when one sigma factor is artificially overexpressed and the effect of this overexpression on growth.

Introduction:

Transcription is the process of copying the genetic information from the template strand of the DNA (deoxyribonucleic acid) to RNA (ribonucleic acid). The enzyme complex involved in this process is called RNA polymerase. It consists of a protein that is involved in the polymerization of RNA (core enzyme) and a protein needed to initiate transcription (sigma factor). Sigma factor helps RNA polymerase to recognize the transcription start site or the promoter site.

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Discuss the following argument: “if the expression of every gene depends on a set of transcription regulators, then the expression of these regulators must also depend on the expression of other regulators, and their expression must depend on the expression of still other regulators, and so on. cells would therefore need an infinite number of genes, most of which would code for transcription regulators.” how does the cell get by without having to achieve the impossible?

The protein NK2 is a transcriptional regulator that functions both as a repressor and activator. To map the two domains more precisely a research group introduced deletion mutations within the coding sequences and studied the effect of a mutation on the NK2 regulatory function. A reporter construct (b-gal activity measurement) was used to monitor gene expression (row 15). Expression in the absence of NK2 was set at 100% (row 15). Repression is indicated by a significant reduction in expression (see row 14 for example) and activation is indicated by higher that 100% activity (e. g., row 11). The presence of the wild type NK2 led to near complete inhibition of expression (row 14). In the figure a deletion is shown as an open area and the residues deleted are indicated on the left (e.g., the row 13 mutant has a deletion of residues 1-97). From the data presented in the Figur, answer the following questions.a) Which region(s) of the protein is required for repression function?b) Is the…

Recombinant expression in prokaryotic systems has numerous advantages when compared to eukaryotic systems, one of which is the ability to produce the protein of interest at high levels. For this, it is essential to use strong promoters and genetically modified bacteria capable of overexpressing the exogenous gene. Therefore, mark the alternative that best represents the set of bacterial promoters/strains for protein overexpression. * A)use of the T7 promoter, whose induction occurs by the addition of IPTG in the culture medium, and use of the Escherichia coli strain BL21DE3. B)use of the T7 promoter, whose induction occurs by the addition of IPTG in the culture medium, and use of the Escherichia coli DH5 strain. C)use of the lac promoter, whose induction occurs by the addition of IPTG in the culture medium, and use of the Escherichia coli DH5 strain. D) use of the constitutive trp promoter, whose induction occurs by the addition of the amino acid Tryptophan in the medium, and use of…

Answer 2

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 8.1, Problem 2TQ

Summary Introduction

To review:

The overall gene expression in the cell when one sigma factor is artificially overexpressed and the effect of this overexpression on growth.

Introduction:

Transcription is the process of copying the genetic information from the template strand of the DNA (deoxyribonucleic acid) to RNA (ribonucleic acid). The enzyme complex involved in this process is called RNA polymerase. It consists of a protein that is involved in the polymerization of RNA (core enzyme) and a protein needed to initiate transcription (sigma factor). Sigma factor helps RNA polymerase to recognize the transcription start site or the promoter site.

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Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 8.1, Problem 2TQ

Summary Introduction

To review:

The overall gene expression in the cell when one sigma factor is artificially overexpressed and the effect of this overexpression on growth.

Introduction:

Transcription is the process of copying the genetic information from the template strand of the DNA (deoxyribonucleic acid) to RNA (ribonucleic acid). The enzyme complex involved in this process is called RNA polymerase. It consists of a protein that is involved in the polymerization of RNA (core enzyme) and a protein needed to initiate transcription (sigma factor). Sigma factor helps RNA polymerase to recognize the transcription start site or the promoter site.

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Check out a sample textbook solution

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Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 8.1, Problem 2TQ

Summary Introduction