Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.3, Problem 2EQ
Summary Introduction
To determine: The percentage of genome that would be left unsequenced if a bacterium has a genome size of 4.1Mb.
Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of the coding as well as the non-coding parts of DNA. DNA sequencing is a widely used method to determine the complete sequence of all the bases present in a DNA molecule. Shotgun sequencing is a type of sequencing technique in which DNA is first fragmented into tiny pieces and then sequencing is done.
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"This study will concentrate solely on the molecular characterization of seaweed samples collected on Panglao island inSouth-Central Luzon, Philippines. Molecular data will be limited to DNA sequences, with identification relying heavily on Genbank returns via blastN. The researchers would obtain their samples from the generated database and analyze the DNA barcode of each macroalgae. The researchers would also compare the previous list of discovered seaweeds from Panglao island to generate a new and updated version for this study. The research would no text end outside or to the neighboring islands around Panglao, Bohol. Neither would the researchers add or include specimens that did not appear in the database or originate from Panglao island. The data to be considered would also not discuss or include the entire taxonomic key of each available sample."
Lab 12- Gel Electrophoresis
If provided an image or sample gel, can you use the MMR/DNA Ladder to predict the size (base
pairs) of unknown sample bands?
If provided an image or sample gel, can you determine if a given individual contains the Alu
insertion? If they are homozygous or heterozygous?
Know the following concepts and terms: wells, comb, buffer, loading dye, FastBlast
What colors are the negative and positive terminals on the electrophoresis chamber?
When a gel is placed in the electrophoresis chamber, which terminal should the wells be closest
to?
What is the charge of a DNA molecule?
Which travels faster, loading dye or your DNA samples?
Which travels farther, large or small pieces of DNA?
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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