Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.3, Problem 3EQ
Summary Introduction
To explain: The results of the study conducted by Venter, Smith, and colleagues.
Introduction: Craig Venter and Hamilton Smith worked on Haemophilus influenza along with their colleagues. They used a technique known as shotgun sequencing for their investigation. Haemophilus influenza is pathogenic bacterium..
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Task 3: Agarose Gel Electrophoresis
Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below.
a. b.
Label each lane of the gel. Write only the corresponding letters in the wells above. Above each band in the size ladder, write its size (in kb).
c.corresponding to the gene.
Approximate the size (in kb) of the polystyrenase gene. Write your answer above the band
Bonus: If you wish to identify the bacterial species in this scenario, what gene is most commonly and routinely sequenced?Answer: __________________________________
Paraphrase and add more information
"This study will concentrate solely on the molecular characterization of seaweed samples collected on Panglao island inSouth-Central Luzon, Philippines. Molecular data will be limited to DNA sequences, with identification relying heavily on Genbank returns via blastN. The researchers would obtain their samples from the generated database and analyze the DNA barcode of each macroalgae. The researchers would also compare the previous list of discovered seaweeds from Panglao island to generate a new and updated version for this study. The research would no text end outside or to the neighboring islands around Panglao, Bohol. Neither would the researchers add or include specimens that did not appear in the database or originate from Panglao island. The data to be considered would also not discuss or include the entire taxonomic key of each available sample."
QUESTION:
what difficulty will you experience if you do genetic manipulation to streptomyces spp. and how can this difficulty overcome ?? how could you modulate the gene expression for improving the productivity of an antibiotic produced by the streptomyces strain ?
discuss with diagram
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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