Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 µm deep. (a) (b) - (c) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this material, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. The new product is a success, so the company decides to further develop the device and manufacture it in a polymer using injection moulding. Describe the fabrication process in this case along with the rationale behind each step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script.

Choose any two of the topics (experiments) outlined below. Your response should include all of the subheadings mentioned below and also abide by the instructions provided. 1) The Phoebaus A Levene experiment: DNA is a polymer. 2) Poly-U experiment by Marshall Nirenberg and J. Heinrich Matthaei, 1961. 3) Kornberg experiment: Fidelity (accuracy) of synthesis. 4) The Fraenkel-Conrat virus experiment. 5) Meselson and Stahl mode of the DNA replication experiment. 6) The Hammerling experiment: Cells store hereditary information in the nucleus. 7) The transplantation experiment: Each cell contains a full set of genetic instruction. SUBHEADINGS 1. Introduction/Study Problem Introduction. Report on what has been done before and identify the gaps that have arisen from your research question. 2. Hypothesis [the proposed explanation made on the basis of limited evidence as a starting point for further investigations. 3. Possible research question 4. Objectives of the experiment 5. Methodology 6.…

Give a detailed description of how probes are designed and arranged on a resequencing array. Your description should include the size (in base pairs) of the probe, where differences in sequence are located within the probe, and the types of experiments that would use this type of microarray.

Peptide Sequencing Answer numbers 1 and 2. For number 2, show the OVERLAP method.

CCCP is observed to have caused increase the SDH activity in DCIP assay. Explain why?

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…

Please offer a clear explanation of the ChIP assay.  A. What characteristics of these cells are being assessed? Why would this be valuable to a research team? B. Define assay and explain how samples are managed step by step with a clear explanation of each step. C. What type of data is being generated by this experiment?

AGAROSE GEL ELECTROPHORESIS PRELAB ANSWER ALL QUESTIONS 1. Type of nucleotide is one of the factors that influence electrophoretic mobility. a) True b) False 2. Electrophoresis is used for DNA separation and not proteins. a) True b) False 3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis. a)True b)False 4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator. a) True b) False 5. During electrophoresis, DNA fragments collect at the Cathode. a) True b) False

Preparing plasmid DNA (double stranded, circular) for Sanger sequencing involves annealing a complementary, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90°C and then slowly bringing the temperature down to 25°C. Why does this protocol work? What enzyme is used and what other components are required in the sequencing reaction? How does the Sanger method determine the sequence?