Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21, Problem 5TY
Let’s suppose you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following statements is the most accurate?
- a. Do PCR to clone the gene because it is much faster.
- b. Do PCR to clone the gene because it is very specific and gives a high yield.
- c. You can’t do PCR because you can’t make forward and reverse primers.
- d. Do cloning using a vector because it will give you a higher yield.
- e. Do cloning by insertion into a vector because it is easier than PCR.
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Match the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template
A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR?
A. PCR is more likely to have errors introduced in the copying process.
B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR.
C. PCR can only be conducted in eukaryotic cells.
D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe?
a. The reaction will work, but at a significantly slower rate.
b. The reaction will work, but the product will contain many undesired mutations.
c. The reaction will work, but amplify a region that was not his target.
d. The reaction will be completely unsuccessfu
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What would be the effect of performing a RT-PCR with the following ingredients: an mRNA template, appropriate primers, dNTPs, heat-stable reverse transcriptase and human DNA polymerase? Select one: a. The PCR would occur, but with a high mutation rate b. The PCR reaction will not commence c. Non-specific PCR of random templates will occur d. The RNA template would be converted to DNA, but the DNA segment would not be amplified PCR would proceed normally е.arrow_forwardWhich of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forwardSuppose that you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following is the most accurate? O Perform a PCR to clone the gene because it is much faster. Perform a PCR to clone the gene because it is very specific and gives a high yield. O PCR cannot be performed because the DNA sequence is not known yet so the forward and reverse primers cannot be designed. O PCR can be performed because although the DNA sequence of the sample is not known yet, an available primer can still be used.arrow_forward
- What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardDescribe the method of a PCR technique in which you can amplify fragments randomly? Briefly.arrow_forward
- When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The temperature where the DNA molecules denature. b.The temperature of the first step in a PCR cycle. c.The temperature where half of the DNA molecules are denatured. d.Tm is the same as the annealing temperature. e.The Temperature at which all of the DNA molecules are dentaturedarrow_forwardYou are tasked with amplifying a gene of interest with PCR. a. After performing a Southern blot, you see no signal on your gel. What could have happened with your PCR? b. After troubleshooting your PCR, you now want to perform Reverse Transcriptase (RT) PCR to look at MRNA expression. Assuming the primers in 2a were not the problem for your DNA and using the same primers as 2a, you see no signal on your Southern blot. What are possible causes?arrow_forwardFor each of the following experimental goals, is PCR orgene cloning preferable and why?a. Isolate the same gene from 20 individuals.b. Isolate 100 genes from the same individual.c. Isolate a mouse gene when you have a rat genefragment.arrow_forward
- Choose the correct statements from the list below. There may be more than one correct statement. A) If you start with 2 DNA templates, after four rounds of PCR you'll have 32 copies B) PCR is useful in making millions or billions of copies of a gene so that it is present in a quantity large enough to study C) quantitative PCR is very similar to PCR, but fluorescent probes are added so that we can measure how much PCR product exists by examining how much the reaction fluoresces D) In real-time reverse transcriptase PCR, the RNA is used as a template to make a cDNA copy (through reverse transcriptase)arrow_forwardWhat is the improtance of creating the master mix in the PCR lab? Pick all that apply. a. It makes the reaction run faster. b. It ensures more consistent concentrations of reagents for each tube. c. It helps correct for pipette errors at small volumes. d. It is easier to set up a single tube to add needed components in bulk versus pippetting each sample tube individually.arrow_forwardYou have been performing a PCR reaction but your results aren't the greatest. Your Supervisor has told you that you should increase the concentration of Magnesium. What affect will this have on the reaction? a. The annealing temperature will not be affected but the enzyme activity will be affected. b. The annealing temperature will decrease. c. The denaturation temparture will have to be increased in the PCR protocol. d. The denaturation temparture will have to be decreased in the PCR protocol. e. The Annealing temperature will increase.arrow_forward
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