Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.2, Problem 3CC
Summary Introduction
To explain: The way in which sgRNA is different from certain components of the bacterial defense system.
Introduction: CRISPR-Cas system is a system used by the bacterial defense mechanism. However, this system can be used to modify the genes. This method of using a CRISPR-Cas system to alter the genes in an organism is known as CRISPR-Cas technology. sgRNA stands for “single guide RNA”. It is used in CRISPR-Cas technology. sgRNA is produced in such a way that it is complementary to the target gene. Therefore, it becomes easy for the researchers to locate the target gene from the complete DNA strand.
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Students have asked these similar questions
True or False.
1. a.) RNA polymerase decodes mRNA so the ribosome can make proteins.
b.) Only coding RNA can interact with the ribosome.
c.) The ribosome is composed of both protein and ncRNA.
d.)The ncRNA components of the ribosome behave as a ribozyme.
Pick one of the FALSE statements from the 4 previous questions and explain why it is incorrect.
Q. When dsRNA is treated with Dicer enzyme siRNA and miRNA’s are produced. What role they will play in gene regulation and expression and how? (Subject Bioinformatics)
INSTRUCTION:
= IF BOTH STATEMENT ARE TRUE
= IF FIRST STATEMENT IS TRUE WHILE SECOND STATEMENT IS FALSE
= IF FIRST STATEMENT IS FALSE WHILE SECOND STATEMENT IS TRUE
= IF BOTH STATEMENTS ARE FALSE
STAMENT 1: RNA splicing is the step in post transcriptional processing where intervening sequences are removed
STAMENT 2: 5’ to 3’ direction is the direction of growth of the peptide chain
ANSWER:
STAMENT 1: The enzyme that joins the gaps in newly synthesized DNA is called DNA polymerase
STAMENT 2: The name of the compound formed when cytosine is bonded to ribose is cytidine
ANSWER:
STAMENT 1: Codon is a term that refers to the 3-nucleotide code for amino acids in mRNA
STAMENT 2: Transition is a kind of mutation where a purine changes to another purine
ANSWER:
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- . What is the purpose for generating the dsRNA? What will it be used for in the lab?arrow_forwardRecall from the central dogma that DNA codes for mRNA, which then codes for protein. Also recall that directionality matters! DNA 3' TAC - CTA -AAT - TGC - TCG-ATT 5' mRNA 5' ???- ???- ???- ???- ???- ??? 3' protein ? ? ? ? ? (A) Indicate whether the DNA sequence provided is the sense strand or the antisense strand. ? that (B) For the DNA sequence given above, write out the mRNA sequence that results. (C) Now write the amino acid sequence that results from the mRNA sequence you wrote in part (B). Use the three-letter abbreviations for the amino acids. (D) What happens if the A that is bolded and underlined in the given DNA sequence is mutated (changed) to a C? How is the protein affected? This can be answered in a few words, but be specific! (E) Now let's pretend for a moment that the protein being affected is ATP-ADP translocase. What, if anything, would happen to the citric acid cycle? This should be answered in a few words/one sentence max.arrow_forwardImagine Cas9 used a 30 base RNA molecule instead of a 20 base molecule. How often will it cut? Hint: each base (A,G,T,C) is equally likelyarrow_forward
- Need help:, The rRNAs are isolated from the large subunit of a bacterial ribosome and separated by density gradient centrifugation. Draw the resulting density gradient and label the bands observed. Which rRNA is longest?arrow_forwardQ1: What common mechanism is employed by the guide RNA to find its target DNA sequence? Q2: How many strands of DNA must Cas9 cut to be effective? Q3: Does Cas9 also cause the deletion of DNA from the genome?arrow_forwardQuestion:- Define the transcription unit. How does it differ from the gene? Describe how you would determine the 5' and 3' ends of a transcription unit in a genome browser.arrow_forward
- INSTRUCTION: = IF BOTH STATEMENT ARE TRUE = IF FIRST STATEMENT IS TRUE WHILE SECOND STATEMENT IS FALSE = IF FIRST STATEMENT IS FALSE WHILE SECOND STATEMENT IS TRUE = IF BOTH STATEMENTS ARE FALSE STAMENT 1: UGA, UAG and UCG are termination codon STAMENT 2: Missense mutation is a type of mutation that changes the coded amino acid ANSWER: STAMENT 1: Binding of RNA primer to the DNA is the first step in the transcription cycle STAMENT 2: Translation refers to the synthesis of proteins using the information contained in mRNA ANSWER: STAMENT 1: The carbon number in ribose where guanine is connected is 1 STAMENT 2: The other name for unprocessed eukaryotic RNA is raw nuclear RNA ANSWER:arrow_forwardPlease answer fast Explain how you would go about developing new ribozymes capable of targeting new sequences and that can be controlled using effector molecules of your choosing.arrow_forwardMAKE CONNECTIONS Compare the CRISPR-Cas systemto the miRNA system discussed in Concept 18.3, includingtheir mechanisms and their functions.arrow_forward
- Explain the Gene Expression Experiment Re-Analysis Summary of Rabies Virus(Taxon:11292) Explain how The virus attaches to the cell membrane of the host cell via the G protein. How the virus penetrates the cytoplasm and the ribonucleoprotein (RNP) complex is exposed to the cell's machinery. Explain Phylogenetic Analysis and Adenovirus Vaccine Cloning and Rationalization of Rabies Virus.arrow_forward32. CRISPR #2: The CASCADE-DNA structure reveals the configuration of the crRNA- DNA duplex but it does not show how the subsequent nuclease DNA degradation step occurs. This DNA degradation step requires the Cas3 helicase-endonuclease enzyme. Cas3 is subsequently able to unwind and degrade the invading DNA. How does Cas3 specifically target the invading DNA?arrow_forwardGive typed explanation Use the diagram representing the typical steps in gene expression and protein formation to respond. All of the following help the cell regulate gene expression during Step 2 of protein formationexcepta)general factors that are required for the RNA polymerase to bind to the promoter.b)repressors binding to the operator preventing the RNA polymerase from progressing.c)specialty factors binding to the RNA polymerase, altering its specificity.d)tRNA that binds to the RNA polymerase in the presence of complementary mRNA.arrow_forward
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