Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 21, Problem 6TY
In the CRISPR-Cas technology for editing genes, what is (are) the function(s) of sgRNA?
- a. to bind to the target gene
- b. to bind to Cas9
- c. to cause a double-strand break in the target gene
- d. all of the above
- e. both a and b
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Students have asked these similar questions
Using CRISPR/Cas9 to target a gene in the mouse will primarily result in:
a. Any of the choices
b. Synonymous substitutions
C. Homozygous gene knockouts
d.
Missense mutations
e. Frameshift mutations
How can Cas9 technology be used in gene therapy.
A. Base editing can be used to repair DNA error
B. All 3 answers
C. Cas9 can be used to activate transcription of a gene
D. Knocking out a gene if it’s gain a function mutation is the cause of the disease
1. a. CRISPR-Cas9 is a valuable biotechnology tool. Explain how this system uses RNA and the result of the interaction.
b. How is this different from miRNA?
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- Which of the following types of transposable elements rely onan RNA intermediate for transposition?a. Insertion elementsb. Simple transposonsc. Retrotransposonsd. All of the abovearrow_forwardIn the triplet-binding assay of Nirenberg and Leder, an RNA triplet composed of three bases was able to cause the a. translation of a polypeptide. b. binding of a tRNA carrying the appropriate amino acid. c. termination of translation. d. release of the amino acid from the tRNA.arrow_forwarda. What is grey donut shaped object supposed to represent? b. Label with the word "promoter" where one might find the promoter region in this image. c. In the magnified insert, label the template strand that is being transcribed with the word "template". d. In the magnified insert label the 5' and 3' ends of the transcript. e. What entity functions to break the complementary base pairing of a gene at its transcription start site? CS Scanned with CamScannerarrow_forward
- Complete the following sentence: Transcription of DNA by RNA polymerase II synthesises microRNA precursor transcript in: A. either the 3′ to 5′ direction or the 5′ to 3′ direction depending on the orientation of the gene B. a discontinuous fashion. C. the 3′ to 5′ direction. D. both the 3′ to 5′ direction and the 5′ to 3′ direction simultaneously E. the 5′ to 3′ direction.arrow_forwardWhich of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.arrow_forwardWhich of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.arrow_forward
- Describe the following a. Core enzyme RNA polymerase b. Sigma factor c. Promoter consensus sequence d. Rho Dependent Factor e. Intrinsic Factor f. Abortive Initiationarrow_forwardA peptide is produced in two different cells. The primary structure is the same in both cells, but the amount of peptide is increased in one cell. What could be happening to the pre-mRNA or mRNA transcript within the cell that has the higher amount of this peptide? A. It has a shorter Poly-A tail B. An alternative polyadenylation (Poly-A) site is being selected C. Leaky scanning occurred D. B and C are both correct OE. None of the abovearrow_forwardChoose the item in column 2 that best matches each item in column 1. A. Provides information for production of protein B. Binds to promoter C. Promoter has a box A and box B consensus sequences D. Autocatalytic RNA molecules E. Associated with transcription termination F. Polymerization of ribonucleotides to form RNA molecules G. 7-methyl guanosine H. MRNA prior to processing select - 1. Core RNA polymerase select 2. rho (p) factor select 3. hnRNA select - 4. RNA select -5. RNA polymerase holoenzyme select 6. Ribozymes select - 7. 5' MRNA cap select 8. MRNAarrow_forward
- Alternative RNA splicing a. is a mechanism for increasing the rate of transcription. b. can allow the production of proteins of different sizes from a gene. C. can allow the production of similar proteins from different RNAS. d. increases the rate of transcription. e. is due to the presence or absence of particular snRNAs.arrow_forwardProvide the SIGNIFICANT differences between the following: 1. Regulation AFTER transcription mRNA processing miRNAs a. b. 2. Types of Genes Facultative Constitutive a. b.arrow_forwardIn gene silencing, the “dicer” enzymea. assembles siRNAs into a RISC complex.b. unwinds the RISC complex.c. binds siRNA to mRNA sequences complementary to it.d. slices up double-stranded RNA moleculesarrow_forward
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