What is the purpose for generating the dsRNA? What will it be used for in the lab?

Q: Summarize the difference between a short tandemrepeat and a transposon.

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: RNAi...  result in product of dsrna or sirna to sirna or mirna! does it increase or decrease…

A: RNA i or RNA interference is a natural process occurs; in most of the eukaryotes;post…

Q: draw the p21 promoter. Your drawing should include (1) the start site, (2) the TATA box and (3) the…

A: p21 protein:  The p21 protein is a key protein that regulates the DNA repair process inside the…

Q: My Avya mouse is brownish because a) the CpG island with the transposon is unmethylated. b) it was…

A: Epigenetic systems that facilitate gene-environment interactions are involved in the ability of…

Q: Define the somatic gene therapy ?

A: Every living organism has Deoxyribonucleic acid (DNA). DNA has a recipe for synthesizing proteins.…

Q: How does RNA polymerase “know” where to start transcribing a gene?

A: RNA polymerase- It is the most important enzyme of transcription process, this synthesizes RNA from…

Q: Describe the function in prokaryotic transcription of theRNA polymerase core enzyme, sigma (σ)…

A: The first step in gene expression is the transcription, which is a common phenomenon in both…

Q: What two processing steps ensure that RNA is stabilized before it leave the nucleus?

A: RNA encodes amino acids sequences  and coveys genetic information from DNA to the translation…

Q: What are the profiled epigenomic modifications found from the epigenome roadmap programme

A: Introduction:- Heritable chemical or physical alterations in chromatin are known as epigenetic…

Q: Outline the steps that occur when piRISCs silence transposable elements by repressing transcription…

A: Piwi-interacting RNA (ribonucleic acid) or piRNA refers to the small non-coding RNA that is…

Q: What is the initiation complex and how it is different from the preinitiation complex?

A: Initiation complex - All the components required for the the initiation of translation process is…

Q: What steps involved in formation of a pre-initiation complex (PIC) ?

A: Pre-initiation of complex is a complex of proteins formed in the eukaryotic cells. The transcription…

Q: Outline the goals and major findings of the ENCODE Project.

A: ENCODE stands for "Encyclopedia of DNA Elements". It was a research project that came after the…

Q: To inactivate a gene by RNAi, what information do youneed? Do you need the map position of the…

A: RNA interference is a simple method uses for silencing a gene expression. During this process, the…

Q: Q1: What common mechanism is employed by the guide RNA to find its target DNA sequence? Q2: How many…

A: In molecular biology, DNA is called as Deoxyribonucleic acid and RNA is called as Ribonucleic acid.…

Q: Explain next-generation sequencers.

A: Sequencing is the process that helps to find out the primary structure of a biopolymer.…

Q: B. Describe in complete sentences how you would identify a target DNA cleavage site for CRISPR-Cas9…

A: A target DNA cleavage site would have PAM sites - 5' NGG flanking both sides of the target.In the…

Q: 32. CRISPR #2: The CASCADE-DNA structure reveals the configuration of the crRNA DNA duplex but it…

A: The main function of cas3 is to cleave the targeted invading DNA.  When alien DNA enters cell,…

Q: Illustrate the crRNA biogenesis ?

A: The mature form of crRNAs is important in CRISPR-Cas defense against genome pathogens. These are the…

Q: Briefly, how can we have so many TCRs and BCRs (billions!) with only 100 genes dedicated to making…

A: The T-cell receptor (TCR) is a protein complex present on the exterior of T cells, also known as T…

Q: An ncRNA may have the following functions: scaffold, guide, alterer of protein function or…

A: Introduction  RNA stands for Ribose Nucleic Acid; it also serves as genetic material in many species…

Q: . You have identified a mutation in yeast, a unicellular eukaryote, that prevents the capping of the…

A: Central dogma states the conversion of DNA into mRNA via transcription and mRNA into proteins by…

Q: What are the differences between hnRNAs, snRNAs, miRNAs, siRNAs, and snoRNAs?

A: hnRNAs, snRNAs, miRNAs, siRNAs, and snoRNAs are the different classes of small RNAs involved in…

Q: Define about Amplified cDNA fragments ?

A: Amplification is a process in which the the single DNA can be sequenced to get numerous DNA…

Q: Identify enhancers with GFP reporters?

A: Enhancers are highly parallel in identification of active sites, and they are very tissue-specific.…

Q: Discuss transposons and their importance to microorganisms.

A: Genetics is a branch of science that deals in the study of genes, heredity, and genetic variation of…

Q: RNA processing events include A. self splicing B. methylation of 45S FRNA C. snoRNA facilitates…

A: Introduction: RNA processing involves newly transcribed RNA molecules (primary transcript)…

Q: The transcripts from dispersed core promoter type genes will a) start from a defined single site b)…

A: A. The RNA polymerase II center advertiser is for the most part characterized to be the grouping…

Q: Explain how the CRISPR-Cas system defends bacteria against bacteriophages.

A: A bacteriophage is a virus that specifically infects bacteria. These viruses take over the machinery…

Q: An investigator designed a crRNA to target a gene with sequence GAATGCTATGCTTAGATTAG. Please very…

A: A crucial part of the CRISPR-Cas gene editing system is played by the short RNA molecule known as…

Q: a few sentences: Describe in detail the 3 steps of transcription and the 3 steps of translation.…

A: 3 steps of Transcription Initiation ElongationTerminationIn Initiation, the RNA polymerase binds to…

Q: Describe how transposable elements alter the genome

A: Transposable element also known as the jumping gene is a DNA sequence that is capable to change its…

Q: Imagine the four basic components of gene regulation are DNA, RNA, proteins, and small molecules.…

A: DNA and RNA are self-replicating genetical materials. Various types of RNAs are transcribed from…

Q: An investigator designed a crRNA to target mouse myostatin gene with sequence TGACGATTATCACGCTACCA.…

A: CRISPR RNA (crRNA) is a short RNA molecule that plays a key role in the CRISPR-Cas gene editing…

Q: Describe the three models for RNAP active-center translocation during transcription initiation?

A: In gene expression. transcription initiation is the first and highly regulated process. In initial…

Q: Design a crispr crRNA portion of the gRNA for targeting myostatin gene in mo or tracerRNA which…

A: designing an effective gRNA for CRISPR-Cas9 gene editing requires careful consideration of various…

Q: Enhancer sequences may be very far from the genes they affect but they are always upstream of the…

A: Enhancer sequence : These are regulatory DNA sequences, when these are bound by transcription…

Q: Describe the methods used to identify the location of transcription control elements in promoter…

A: The sequence of nucleotides in a gene that controls gene transcription is known as a transcriptional…

Q: How does a genomic library differ from a cDNA library?

A: The whole genome of an organism can be stored in the form of a DNA library. DNA libraries contain…

Q: Transposable elements alter the genome by making what changes to genes?

A: Transposition may be related to replication, recombination, and repair. Insertion of transposable…

Q: How to Find gene-regulatory regions, such as promoters andenhancers ?

A: One of the most difficult tasks facing scientists in the post-genomic age is unravelling the…

Q: Q. Deletion of a single AT base pair from codon number 4 can cause a frameshift mutation in a…

A: The DNA is transcribed into to an RNA by the process of transcription. Following the transcription…

Q: When dsRNA is treated with Dicer enzyme siRNA and miRNA’s are produced. What role they will play in…

A: siRNA is a type of double stranded short sequence rna which is non coding and take part in RNA…