Define the transcription unit. How does it differ from the gene? Describe how you would determine the 5' and 3' ends of a transcription unit in a genome browser.

True/False: In general, weak promoters that support active transcription have -10 and -35 boxes that are close to the consensus sequences and a spacer between the two boxes that is 17 +/- 1 bp.

What is "wobble" controlled during transcription in a prokaryotic cell? Explain briefly.

INSTRUCTION: = IF BOTH STATEMENT ARE TRUE = IF FIRST STATEMENT IS TRUE WHILE SECOND STATEMENT IS FALSE = IF FIRST STATEMENT IS FALSE WHILE SECOND STATEMENT IS TRUE = IF BOTH STATEMENTS ARE FALSE   STAMENT 1: UGA, UAG and UCG are termination codon STAMENT 2: Missense mutation is a type of mutation that changes the coded amino acid   ANSWER:   STAMENT 1: Binding of RNA primer to the DNA is the first step in the transcription cycle STAMENT 2: Translation refers to the synthesis of proteins using the information contained in mRNA   ANSWER:   STAMENT 1: The carbon number in ribose where guanine is connected is 1 STAMENT 2: The other name for unprocessed eukaryotic RNA is raw nuclear RNA   ANSWER:

Shown below is a schematic drawing of a gene, with the transcription unit divided into numbered regions. The arrows (;) indicate transcription initiation sites, "D" indicates a splice donor site, "A" indicates a splice acceptor site, and "An" indicates a polyadenylation signal. Give all the possible fully processed mRNAs that could be produced from transcripts of this gene (you don't need to draw anything, just list the regions that would be included in each mRNA by number).

You just isolated a mutant in the eukaryotic organism C. elegans (aka the world's coolest model organism) that fails to transcribe your favorite gene. Which of the following region(s) are likely to contain your mutation? (You may choose more than one.) Group of answer choices Rut site -10 consensus sequence Rho protein TATA box Sigma subunit binding site Transcription start site

Please explain: Transcription-what is it and what does it involve? What happens at initiation, elongation, and termination?

Suppose you have a 1-kb segment of cloned DNA that is suspected to contain a eukaryotic promoter including a TATA box, a CAT box, and an upstream GC-rich sequence. The clone also contains a gene whose transcript is readily detectable. Your laboratory supervisor asks you to outline an experiment. (1) determine if eukaryotic transcription factors (TF) bind to the fragment and, if so, (2) identify where on the fragment the transcription factors bind. All necessary reagents, equipment, and experimental know-how are available in the laboratory. Complete the outline of an experiment, which determines if eukaryotic transcription factors (TF) bind to the fragment. Assume all necessary reagents, equipment, and experimental how are available in the laboratory. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. For the band shift assay, two samples of the DNA fragment are analyzed by agarose gel electrophoresis: one sample is…

You have recently cloned multiple cDNAs for a gene you are studying. After sequencing ten of the cDNAs, you find that seven of the cDNAs would produce a protein of 531 amino acids, while the other three cDNAs would produce a protein missing 30 amino acids from the center of the protein. This finding is can be explained by: O nonsense suppression O alternative splicing O basepair wobble O variability in the length of the poly(A) tail

Both question correlate with each other