Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 20, Problem 9PDQ
What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
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What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction enzyme HaeIII produce “sticky” ends or “blunt” ends?
What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When would you use such enzymes?
Describe two features that are characteristic of the action of restriction endonucleases. How do these features make restriction endonucleases useful to genetic engineers?
Chapter 20 Solutions
Concepts of Genetics (12th Edition)
Ch. 20 - A plasmid that is both ampicillin and tetracycline...Ch. 20 - You have just created the worlds first genomic...Ch. 20 - What undesirable or unforeseen consequences might...Ch. 20 - Do we have the ethical right to alter the genomes...Ch. 20 - Should these new technologies be regulated...Ch. 20 - HOW DO WE KNOW? In this chapter we focused on how...Ch. 20 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 20 - What roles do restriction enzymes, vectors, and...Ch. 20 - The human insulin gene contains a number of...Ch. 20 - Although many cloning applications involve...
Ch. 20 - Using DNA sequencing on a cloned DNA segment, you...Ch. 20 - Restriction sites are palindromic; that is, they...Ch. 20 - List the advantages and disadvantages of using...Ch. 20 - What are the advantages of using a restriction...Ch. 20 - In 1975, the Asilomar Conference on Recombinant...Ch. 20 - In the context of recombinant DNA technology, of...Ch. 20 - If you performed a PCR experiment starting with...Ch. 20 - Prob. 13PDQCh. 20 - Prob. 14PDQCh. 20 - You have recovered a cloned DNA segment from a...Ch. 20 - Prob. 16PDQCh. 20 - Although the capture and trading of great apes has...Ch. 20 - Prob. 18PDQCh. 20 - Prob. 19PDQCh. 20 - Prob. 20PDQCh. 20 - Traditional Sanger sequencing has largely been...Ch. 20 - How is fluorescent in situ hybridization (FISH)...Ch. 20 - What is the difference between a knockout animal...Ch. 20 - Prob. 24PDQCh. 20 - When disrupting a mouse gene by knockout, why is...Ch. 20 - Prob. 26PDQCh. 20 - Prob. 27PDQCh. 20 - As you will learn later in the text (Special...Ch. 20 - The gel presented here shows the pattern of bands...Ch. 20 - A widely used method for calculating the annealing...Ch. 20 - Most of the techniques described in this chapter...Ch. 20 - In humans, congenital heart disease is a common...Ch. 20 - The U.S. Department of Justice has established a...Ch. 20 - Prob. 34ESP
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- What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it cleave? Be as specific aspossible.arrow_forwardFor a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?arrow_forwardHow are restriction endonuclease enzymes named? Write examples.arrow_forward
- What is the function of a restriction enzyme?arrow_forwardIn an attempt to clone a gene into the TOL plasmid, it was observed that the restriction product of the gene of interest were blunt ended, thereby reducing the ligation efficiency of this gene and the TOL plasmid. Elucidate two methods that could be employed to increase the ligation efficiency of your restriction products.arrow_forwardHow to determine the location of restriction sites for different enzyme ?arrow_forward
- Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?arrow_forwardWhat normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes?arrow_forwardWhich of the following can be termed as a restriction modification system?a) Restriction endonuclease + methylaseb) DNA ligase + methylasec) Restriction endonuclease + acetylased) DNA ligase + acetylasearrow_forward
- RFLP's result from mutations in: 1) genes which specify restriction enzymes 2) restriction enzyme recognition sites 3) genes which specify plasmids 4) monomorphic genesarrow_forwardRefer to the diagram of pUC18 (Fig.) to determine which restriction enzymes you could use to insert a gene that would interfere with production of β-galactosidase by the host cell.arrow_forwardIf restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this? Indicate the answerarrow_forward
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