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Curren'S Math For Meds: Dosages & Sol

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 8.5P

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Question

For a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?

a
b
In an organism with 20% AT it will cut, on average, every 256 bases (0.2-4).
с
d
In an organism with 50% GC it will cut, on average, every 256 bases(0.25—4).
e
In an organism with 20% GC it will cut, on average, every 39 bases. (0.44)
A and C
All of the above are True

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. An Hfr+ strain is used to map three genes: ala, leu, and thr in an interrupted mating experiment. Hfr/ala+, leu+, thr+, camS x F-/ala-, leu-, thr-, camR The cross is initiated at time = 0, and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium with chloramphenicol and the amino acids as listed in the table. The data represents the number of colonies growing on each plate. Time of interruption Nutrients added 5 min 10 min 15 min 20 min alanine, leucine 8 21 58 85 leucine, threonine 0 3 19 46 alanine, threonine 0 0 7 16 Based on these data, what is the order of gene transfer relative to one another and to the F-factor.
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An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?
. a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+, Xyl+,Tyr+, Arg+, His+, Mal+, and Strs. Describe anappropriate bacterial strain to be used as theother partner in this mating.b. In an Hfr × F− cross, the pyrE gene enters therecipient in 5 minutes, but at this time point thereare no exconjugants that are Met+, Xyl+, Tyr+,Arg+, His+, or Mal+. The mating is now allowed toproceed for 30 minutes and Pyr+ exconjugants areselected. Of the Pyr+ cells, 32% are Met+, 94% areXyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and71% are Mal+. What can you conclude about theorder of the genes?
You are studying a microorganism that contains a chloramphenicol acetyltransferase (CAT) enzyme, and looking for a clone of this microorganism that no longer contains the gene encoding CAT. Imagine you had an LB plate containing chloramphenicol. You streak an isolated colony of the Parent Strain and Clone A onto the same plate. Which of the following statements are true about the growth pattern after 24 hours in the incubator? Select all that apply? L-hlareeheticol LB chloremphenicel Parent Clune A Parent Clone A LD chlerephenicol 18 chloremphenitol Paremt Clone A Paront Clone A Plate A is the expected growth pattern as clone A should grow in the LB plate with chloramphenicol Plate B is the expected growth pattern as LB plate with chloramphenicol parent strain should grow in the Plate B is the expected growth pattern as clone A should not grow in the LB plate with chloramphenicol Plate A is the expected growth pattern as the parent strain should not grow in the LB plate with…
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.
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Linkage maps in an Hfr bacterial strain are calculated in units of minutes (the number of minutes between genes indicates the length of time that it takes for the second gene to follow the first in conjugation). In making such maps, microbial geneticists assume that the bacterial chromosome is transferred from Hfr to F − at a constant rate. Thus, two genes separated by 10 minutes near the origin end are assumed to be the same physical distance apart as two genes separated by 10 minutes near the F −attachment end. Suggest a critical experiment to test the validity of this assumption.
DNA from a strain of Bacillus subtilis with genotype a* b* c* d* e* is used to transform a strain with genotype a b c d e. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformatic a* and b* No b* and d* No a* and c* No b* and e* Yes a* and d* Yes c* and d* No a* and e* Yes c* and e* Yes b* and c* Yes d* and e* No On the basis of these results, what is the order of the genes on the bacterial chromosome?
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