The special feature of the DNA polymerase enzyme used in PCR. Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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a) (iii) (iv) Polymerase chain reaction (PCR) is a technique that enables multiplication of specific DNA sample at minute amount to millions or billions of copies at a short time span. (i) Figure 1 indicates the chemicals added into the PCR reaction tube prior to the addition of thermostable DNA polymerase. Do you agree with the list? Justify your answer. DNA template Buffer (containing Tris-HCI, KCI, Mg2+) ddNTP Forward primer Reverse primer Figure 1 If TA cloning is planned to be carried out after amplification of the gene, which thermostable DNA polymerase will you select and the reason for your selection? Why is the optimal annealing temperature vital in this technique? Explain how this temperature (too high or too low) will affect the efficiency of this reaction. If the primers you purchased possessed the following information: Number of Guanine: 4 Number of Adenine: 3 Number of Thymine: 4 Number of Cytosine: 5 Calculate the melting temperature of this primer and estimate the…

Put the steps of one PCR cycle in the correct order: The PCR reaction mixture is heated to about 70 degrees, which is the optimum temperature for the polymerase to build the new strands of DNA, starting at the 3' end of the primer. The PCR reaction mixture is heated to 95 degrees Celsius, which denatures the double stranded template DNA. The PCR reaction mixture is cooled to about 50-55 degrees, which allows the primers to find their complementary site on the template and "anneal" the

Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.

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Question

Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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Question

Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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Question

Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

Answer 6

Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

Answer 7

Chapter 20, Problem 20PDQ

Summary Introduction

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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The special feature of the DNA polymerase enzyme used in PCR. Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

Solution Summary: The author explains the special feature of the DNA polymerase enzyme used in PCR.

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To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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Chapter 20 Solutions

The special feature of the DNA polymerase enzyme used in PCR. Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

Solution Summary: The author explains the special feature of the DNA polymerase enzyme used in PCR.

Concept explainers

Videos

To determine: The special feature of the DNA polymerase enzyme used in PCR. Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.

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Chapter 20 Solutions

To determine: The special feature of the DNA polymerase enzyme used in PCR.

Introduction: Polymerase chain reaction (PCR) is a laboratory technique used to make multiple copies of a particular region of DNA. PCR involves three steps: denaturation, annealing, and extension.