Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 4EQ
Summary Introduction
To review:
The experimental results of the data shown.
Introduction:
Promoter bashing is a genetic technique used to identify the genetic sequences, which play an important role in the regulation of transcription of a gene. This method uses a clone which contains a coding region of a gene and a region upstream from the core promoter, which has regulatory elements like silencers and enhancers. The effect of promoter bashing on transcription can be easily determined by swapping the coding gene with lacZ (lac operon) gene, which codes for beta-galactosidase whose activity can be measured using enzyme assay.
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Chapter 15 Solutions
Genetics: Analysis and Principles
Ch. 15.1 - 1. Combinatorial control refers to the phenomenon...Ch. 15.1 - 2. A regulatory transcription factor protein...Ch. 15.1 - 3. A bidirectional enhancer has the following...Ch. 15.1 - 4. Regulatory transcription factors can be...Ch. 15.2 - 1. A chromatin-remodeling complex may
a. change...Ch. 15.2 - Prob. 2COMQCh. 15.2 - 3. Which of the following characteristics is...Ch. 15.2 - 4. Transcriptional activation of eukaryotic genes...Ch. 15.3 - How can methylation affect transcription? a. It...Ch. 15.3 - 2. The process in which completely unmethylated...
Ch. 15.4 - Prob. 1COMQCh. 15.5 - The overall goal of the ENCODE Project is a. to...Ch. 15.6 - The binding of iron regulatory protein (IRP) to...Ch. 15 - Discuss the common points of control in eukaryotic...Ch. 15 - 2. Discuss the structure and function of...Ch. 15 - 3. What is meant by the term transcription factor...Ch. 15 - What are the functions of transcriptional...Ch. 15 - 5. Is each of the following statements true or...Ch. 15 - 6. Transcription factors usually contain one or...Ch. 15 - Prob. 7CONQCh. 15 - Prob. 8CONQCh. 15 - 9. Let’s suppose a mutation in the glucocorticoid...Ch. 15 - Prob. 10CONQCh. 15 - Prob. 11CONQCh. 15 - Prob. 12CONQCh. 15 - 13. Transcription factors such as the...Ch. 15 - An enhancer, located upstream from a gene, has the...Ch. 15 - 15. The DNA-binding domain of each CREB protein...Ch. 15 - The gene that encodes the enzyme called tyrosine...Ch. 15 - Prob. 17CONQCh. 15 - 18. What is a histone variant?
Ch. 15 - Prob. 19CONQCh. 15 - 20. What is meant by the term histone code? With...Ch. 15 - Prob. 21CONQCh. 15 - Histones are thought to be displaced as RNA...Ch. 15 - 23. What is an insulator? Describe two different...Ch. 15 - 24. What is DNA methylation? When we say that DNA...Ch. 15 - Lets suppose that a vertebrate organism carries a...Ch. 15 - 26. What is a CpG island? Where would you expect...Ch. 15 - Describe how the binding of iron regulatory...Ch. 15 - 1. Briefly describe the method of chromatin...Ch. 15 - Researchers can isolate a sample of cells, such as...Ch. 15 - Prob. 3EQCh. 15 - Prob. 4EQCh. 15 - Prob. 5EQCh. 15 - 6. As described in Chapter 21, an electrophoretic...Ch. 15 - Prob. 7EQCh. 15 - 1. Explain how DNA methylation could be used to...Ch. 15 - 2. Enhancers can occur almost anywhere in DNA and...
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