A. The location of silencer and enhancer. B. The reason behind the absence of silencer in the pancreatic cell. D. The reason behind no gene expression in kidney cell. Introduction: A genetic technique called promoter bashing, identifies genetic sequences and promote regulation of gene transcription. A gene which is usually expressed in pancreatic cells is selected for promoter bashing where different segments of the upstream region labeled A to D are deleted, and DNA(Deoxyribonucleic acid) is introduced into the pancreatic cells or kidney cells. The percentage of beta-galactosidase activity is measured in both pancreatic cells and kidney cells.

Question

A patient comes into your clinic exhibiting generic cancer symptoms. In order to help form a diagnosis, you ask about patient history and they tell you that they worked for years in an agricultural job, primarily in handling food for large bovine animals and then later working with insect pesticide treatment of grain fields. You decide to test for elevated levels of the environmental agent(s) in their body and focus your cancer diagnosis on based on the established associations of epigentic effects and cancer. O nickel and cadmium; stomach and skin cancer O benzene; breast, prostate and thyroid cancer O polycyclic aromatic hydrocarbons and benzene; lung, breast, stomach and skin cancer O arsenic and endocrine disruptors; skin, bladder, liver and kidney cancer

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Chapter 15, Problem 5EQ

Summary Introduction

To review:

A. The location of silencer and enhancer.

B. The reason behind the absence of silencer in the pancreatic cell.

D. The reason behind no gene expression in kidney cell.

Introduction:

A genetic technique called promoter bashing, identifies genetic sequences and promote regulation of gene transcription. A gene which is usually expressed in pancreatic cells is selected for promoter bashing where different segments of the upstream region labeled A to D are deleted, and DNA(Deoxyribonucleic acid) is introduced into the pancreatic cells or kidney cells. The percentage of beta-galactosidase activity is measured in both pancreatic cells and kidney cells.

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