Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 29, Problem 8EQ
Prehistoric specimens often contain minute amounts of ancient DNA. What technique can be used to increase the amount of DNA in an older sample? Explain how this technique is performed and how it increases the amount of a specific region of DNA.
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Could actual fi ngerprints taken from human fi ngers be used to perform a DNA fi ngerprint? Explain.
Suppose that you are given a short fragment of DNA to sequence. You amplify the fragment with PCR and set up a series of four dideoxy reactions. You then separate the products of the reactions by gel electrophoresis and obtain the following banding pattern:
Write out the base sequence of the original fragment that you were given. Original sequence: 5′– ________________–3′
Below is an image of the results of a gel electrophoresis experiment.
Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the
DNA size marker (DSM). From this image and by only using Lanes 1-
4 which lane matches each description below?
a) This lane contains the largest DNA fragment:
b) This lane contains the smallest DNA fragment:
c) This lane contains a 2.5 kbp DNA fragment:
42
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12 kb
9.5 kb
6.5 kb
4 kb
2 kb
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Chapter 29 Solutions
Genetics: Analysis and Principles
Ch. 29.1 - Prob. 1COMQCh. 29.1 - Prob. 2COMQCh. 29.1 - 3. A pair of birds flies to a deserted island and...Ch. 29.1 - Prob. 4COMQCh. 29.2 - 1. Phylogenetic trees are based on
a. natural...Ch. 29.2 - Prob. 2COMQCh. 29.2 - An approach that is used to construct a...Ch. 29.2 - 4. Horizontal gene transfer is a process in which...Ch. 29.3 - Prob. 1COMQCh. 29.3 - Prob. 2COMQ
Ch. 29.3 - When the chromosomes of closely related species...Ch. 29 - 1. Discuss the two principles on which evolution...Ch. 29 - 2. Evolution, which involves genetic changes in a...Ch. 29 - Prob. 3CONQCh. 29 - Prob. 4CONQCh. 29 - 5. Would each of the following examples of...Ch. 29 - Distinguish between anagenesis and cladogenesis....Ch. 29 - 7. Describe three or more genetic mechanisms that...Ch. 29 - Explain the type of speciation (allopatric,...Ch. 29 - Prob. 9CONQCh. 29 - Prob. 10CONQCh. 29 - Discuss the major differences among allopatric,...Ch. 29 - Prob. 12CONQCh. 29 - Prob. 13CONQCh. 29 - Would the rate of deleterious or beneficial...Ch. 29 - 15. Which would you expect to exhibit a faster...Ch. 29 - Prob. 16CONQCh. 29 - 17. Plant seeds contain storage proteins that are...Ch. 29 - Take a look at the -globin and -globin amino acid...Ch. 29 - Compare and contrast the neutral theory of...Ch. 29 - Prob. 20CONQCh. 29 - 21. As discussed in Chapter 27, genetic variation...Ch. 29 - Prob. 22CONQCh. 29 - Two populations of snakes are separated by a...Ch. 29 - 2. Sympatric speciation by allotetraploidy has...Ch. 29 - 3. Two diploid species of closely related frogs,...Ch. 29 - A researcher sequenced a portion of a bacterial...Ch. 29 - F1hybrids between two species of cotton,Gossypium...Ch. 29 - 6. A species of antelope has 20 chromosomes per...Ch. 29 - Prob. 7EQCh. 29 - 8. Prehistoric specimens often contain minute...Ch. 29 - From the results of the experiment of Figure...Ch. 29 - InChapter 23, a technique called fluorescence in...Ch. 29 - Prob. 11EQCh. 29 - 12. Discuss how the principle of parsimony can be...Ch. 29 - 13. A homologous DNA region, which was 20,000 bp...Ch. 29 - Prob. 14EQCh. 29 - Prob. 1QSDCCh. 29 - 2. Compare the forms of speciation that are slow...Ch. 29 - 3. Do you think that Darwin would object to the...
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- After DNA fragments have been separated by gel electrophoresis, how can they be visualized?arrow_forwardAn effective DNA probe can sometimes be developed by knowing the amino acid sequence of the protein encoded by the gene. A student argued that this is too time-consuming since the complete amino acid sequence must be determined in order to create the probe. Does the student have a valid argument? Why or why not?arrow_forwardCould actual fingerprints taken from human fingers be used toperform a DNA profile? Explain.arrow_forward
- Use a drawing to illustrate the principle of DNA gel electrophoresis. Indicate roughly the comparative electrophoretic mobilities of DNAs with 150, 600, and 1200 bp.arrow_forward1 2 Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will model the process yourself to solve a crime. Good luck, detective! Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA is shown in Figure 5. 10 50 DNA Profile from Forehead Print Number of base pairs 00 50 40 D58818 075830 I 16 MU DES1179 Shandand (10) 70 CSF1PO DITS820 80 100 Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179). and data for the four STR loci that were included in the A suspect was identified in the case. Her DNA…arrow_forwardSometimes people don’t recover enough DNA from their cheek cells to be visible. Suggest a change to the procedure that could result in more DNA being collected.arrow_forward
- A scientist made mistakes during DNA extraction. On his first attempt, he blended the sample too much for a long time. On his 2nd attempt, he used potassium acetate instead of sodium chloride. On his third attempt, he forgot to put C4H8O2.1. Which attempt would be the highest based on increasing DNA purity? what about by increasing DNA yield?2. Explain the effect of his mistakes in each of his attempts on the yield and purity of the DNA sample extracted.3. Which attempt would be the most successful? Why?arrow_forwardExplain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting.Why does this technique work? Mention restriction enzymes and their function. Keep answer between 2-3 sentencesarrow_forwardBriefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain reaction (PCR). Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.arrow_forward
- From what Human Remains may it be possible to recover DNA?arrow_forwardOne way scientists determine the abundance of different DNA molecules in a sample is by measuring the difference in time it takes to reach the detection threshold for each. Sketch a graph of the number of cycles needed to reach the detection threshold as a function of the initial number of molecules. Comment on the relationship between differences in initial number of molecules and differences in the time to reach the detection threshold.arrow_forwardDefine the method for identifying the DNA pieces ?arrow_forward
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