Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 29, Problem 10CONQ
Summary Introduction
To review:
Whether the phenomenon of reproductive isolation applies to bacteria and division of bacteria into separate species by a geneticist.
Introduction:
A set of organisms that can interbreed and produce a fertile offspring along with common morphological traits, molecularfeatures, evolutionary, and some other characteristics is known as species. Species evolve and give rise to the distinct form by the process known as
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When the interrupted mating technique was used with five different strains of Hfr bacteria, the following orders of gene entry and recombination were observed. On the basis of these data, draw a map of the bacterial chromosome. Do the data support the concept of circularity?Hfr Strain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A B
How does recombination in bacteria differ from recombination in eukaryotes?
1) Recombination does not occur in prokaryotes
2) Only the plasmid DNA goes through recombination in prokaryotes
3) Sister chromatids, not homologous chromosomes, exchange information during recombination in prokaryotes
4) A portion of DNA from genome is replaced with homologous DNA from another strain of bacteria.
DNA from a strain of Bacillus subtilis with genotype a + b + c + d + e + is used to transform a strain with genotype a – b – c – d – e – . Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? Show how you work out the order by diagraming.
Chapter 29 Solutions
Genetics: Analysis and Principles
Ch. 29.1 - Prob. 1COMQCh. 29.1 - Prob. 2COMQCh. 29.1 - 3. A pair of birds flies to a deserted island and...Ch. 29.1 - Prob. 4COMQCh. 29.2 - 1. Phylogenetic trees are based on
a. natural...Ch. 29.2 - Prob. 2COMQCh. 29.2 - An approach that is used to construct a...Ch. 29.2 - 4. Horizontal gene transfer is a process in which...Ch. 29.3 - Prob. 1COMQCh. 29.3 - Prob. 2COMQ
Ch. 29.3 - When the chromosomes of closely related species...Ch. 29 - 1. Discuss the two principles on which evolution...Ch. 29 - 2. Evolution, which involves genetic changes in a...Ch. 29 - Prob. 3CONQCh. 29 - Prob. 4CONQCh. 29 - 5. Would each of the following examples of...Ch. 29 - Distinguish between anagenesis and cladogenesis....Ch. 29 - 7. Describe three or more genetic mechanisms that...Ch. 29 - Explain the type of speciation (allopatric,...Ch. 29 - Prob. 9CONQCh. 29 - Prob. 10CONQCh. 29 - Discuss the major differences among allopatric,...Ch. 29 - Prob. 12CONQCh. 29 - Prob. 13CONQCh. 29 - Would the rate of deleterious or beneficial...Ch. 29 - 15. Which would you expect to exhibit a faster...Ch. 29 - Prob. 16CONQCh. 29 - 17. Plant seeds contain storage proteins that are...Ch. 29 - Take a look at the -globin and -globin amino acid...Ch. 29 - Compare and contrast the neutral theory of...Ch. 29 - Prob. 20CONQCh. 29 - 21. As discussed in Chapter 27, genetic variation...Ch. 29 - Prob. 22CONQCh. 29 - Two populations of snakes are separated by a...Ch. 29 - 2. Sympatric speciation by allotetraploidy has...Ch. 29 - 3. Two diploid species of closely related frogs,...Ch. 29 - A researcher sequenced a portion of a bacterial...Ch. 29 - F1hybrids between two species of cotton,Gossypium...Ch. 29 - 6. A species of antelope has 20 chromosomes per...Ch. 29 - Prob. 7EQCh. 29 - 8. Prehistoric specimens often contain minute...Ch. 29 - From the results of the experiment of Figure...Ch. 29 - InChapter 23, a technique called fluorescence in...Ch. 29 - Prob. 11EQCh. 29 - 12. Discuss how the principle of parsimony can be...Ch. 29 - 13. A homologous DNA region, which was 20,000 bp...Ch. 29 - Prob. 14EQCh. 29 - Prob. 1QSDCCh. 29 - 2. Compare the forms of speciation that are slow...Ch. 29 - 3. Do you think that Darwin would object to the...
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- DNA from a strain of Bacillus subtilis with genotype a* b* c* d* e* is used to transform a strain with genotype a b c d e. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformatic a* and b* No b* and d* No a* and c* No b* and e* Yes a* and d* Yes c* and d* No a* and e* Yes c* and e* Yes b* and c* Yes d* and e* No On the basis of these results, what is the order of the genes on the bacterial chromosome?arrow_forwardCompare and contrast conjugation, transduction, and transformation. Which processes, if any, involve homologous recombination and which, if any, do not?arrow_forwardDo all of them True/False 31) The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.Answer: 32) Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.Answer: 33) In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.Answer: 34) All stem cells have the potential to differentiateAnswer: 35) A bone marrow transplant involves the transfer of multipotent stem cellsAnswer: 36) The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.Answer:arrow_forward
- A technique called fluorescence in situ hybridization (FISH) is described. In this method, a labeled piece of DNA is hybridized to a set of chromosomes. Let’s suppose that you cloned a piece of DNA from G. pubescens and used it as a labeled probe for in situ hybridization. What would you expect to happen if this DNA probe were hybridized to the G. speciosa or G. tetrahit chromosomes? Describe the expected results.arrow_forwardWhat is horizontal gene transfer? What are the three mechanisms for this to occur in bacteria? What are the components needed for the processes of transformation, conjugation, and transduction? How does each process occur? What genes are involved in each process? How do generalized and specialized transduction differ? What is the end result of each? What is recombination? What is the importance to bacteria & archaea? What are the two types of recombination? What are the details of each type? What components are needed for each type?arrow_forwardDNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a– b– c– d– e–. Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? please show how you worked out the order by diagraming, thank you! Pair of genes Co-transformation a+ and b+ no a+ and c+ no a+ and d+ yes a+ and e+ yes b+ and c+ yes b+ and d+ no b+ and e+ no c+ and d+ no c+ and e+ yes d+ and e+ noarrow_forward
- The presence (+) or absence (−) of six sequences in each of five bacterial artificial chromosome (BAC) clones (A–E) is indicated in the following table. Using these markers, put the BAC clones in their correct order and indicate the locations of the numbered sequences within them. Sequences BAC clone 1 2 3 4 5 6 A + − − − + − B − − − + − + C − + + − − − D − − + − + − E + − − + − −arrow_forwardDespite being asexual, bacteria can develop genetic variability during the process of conjugation. An exogenous single-stranded piece of linear DNA can be sent from a donor into a recipient where it reforms double-stranded DNA that lines up next to a plasmid or the recipient's main genome and undergoes crossing over events to incorporate new alleles, or in some cases, entirely new genes. Consider a situation where an exogenote, which contains a series of six mutant LOF alleles, recombines with the donor's endogenote, which contains all wild type alleles, and pick the correct statement. a Recombination will produce a donor bacterium that is fully functional. b Recombination containing an odd number of cross-overs will keep the donor bacterium alive, but it will no longer be able to produce certain gene products since it now contains one of more LOF alleles. c Recombination containing an even number of cross-overs will keep the donor bacterium alive, but it will…arrow_forwardDNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a− b− c− d− e−. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformation a+ and b+ No b+ and d+ No a+ and c+ No b+ and e+ Yes a+ and d+ Yes c+ and d+ No a+ and e+ Yes c+ and e+ Yes b+ and c+ Yes d+ and e+ No On the basis of these results, what is the order of the genes on the bacterial chromosome?arrow_forward
- Sam is working in a genetics lab this summer to gain more hands-on experience before applying to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E. coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr1, Hfr2, Hfr3, Hfr4) to help him determine the gene order. He observed that each Hfr strains transferred genes at the times shown in the table below. However, Sam is a little forgetful and neglected to record the times at which several of the genes were transferred. Afraid that the professor will become angry with him, Sam comes to you for assistance. Using the data from the experiment; fill in the missing data in the table attached and draw a single circular map of the seven genes as they would appear on the E. coli chromosome. Be sure to…arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?arrow_forwardWhat is the meaning of conjugation in biology?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license