Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21.4, Problem 1COMQ
The purpose of site-directed mutagenesis and CRISPR-Cas technology is to
a. determine if a protein binds to a DNA segment.
b. determine the sequence of a segment of DNA.
c. alter the sequence of a segment of DNA.
d. determine if a gene is expressed.
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Students have asked these similar questions
Using CRISPR/Cas9 to target a gene in the mouse will primarily result in:
a. Any of the choices
b. Synonymous substitutions
C. Homozygous gene knockouts
d.
Missense mutations
e. Frameshift mutations
The goal of most gene therapies is to insert a healthy copy of a gene into the genome. Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions.
B. The same gene integrated into different locations in the genome will be expressed differently. Why?
1a) Why is it possible for you to study the eye colour gene by extracting cheek cells?
a. Because the nucleus of every cell in the human body contains the same genetic information.
b. Because the cheek cells are located near the cells of the eye and so they are able to exchange DNA.
c. Because all genes in the human body are expressed at all times so it is easy to study them.
d. All of the above are possible explanations.
1b) What is the purpose of heating the sample to 75°C following addition of the 0.2M NaOH solution?
a. To denature the histone proteins that are keeping the DNA tightly coiled.
b. To ensure that all the DNA is removed from the swab in preparation for PCR.
c. To breakdown the cheek cell membrane to release the DNA from the cell.
d. It breaks down the circular DNA down into linear fragments so that they will be easier to visualize.i
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?arrow_forwardGenome annotation refers to ... 1.) lining up overlapping regions in short shotgun sequencing reads to assemble larger contiguous DNA sequences (contigs/scaffolds). 2.) using long-read sequencing platforms (such as PacBio or Oxford Nanopore) to gather information about the epigenetic status of each region of a sequenced genome. 3.) the process of predicting which parts of a genome sequence code for functional products (such as protein-coding genes), what those products do, and assigning them names. 4.) sequencing messengeRNA measure the relative expression levels of genes in one or more tissue samples.arrow_forwardIntroducing a specific change in the mouse genome using CRISPR/Cas9 requires: a. Alterations to both alleles of a gene b. An HDR gene fragment c. Non-homologous end joining (NHEJ) d. Leaving out the guide RNA e. All of the choicesarrow_forward
- CRISPR is a powerful gene editing tool because a. the programmable enzyme (Cas9) can find an exact sequence and make a cut at precise nucleotide. b. it can be applied to all types of cells. c. it has both genetic editing and epigenetic functions. d. it is inexpensive. e. all of these are correctarrow_forwardPropose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for altering cellular functions. What might these functions be, and how could CRISPR-Cas be used to study them?arrow_forwardTautomerization: A. Causes frameshift insertions B. Causes frameshift deletions C. Results in permanent chemical changes in nitrogenous bases D. Cause transition mutations E. Cause transversion mutationsarrow_forward
- Which of the following is true? a. Enhancers are proteins that bind to activator DNA sequences. b. Activators are proteins that bind to insulator sequences c. Repressors are proteins that bind to enhancer DNA sequences. d. Repressors are proteins which bind to silencer DNA sequencers.arrow_forwardPart a) and b) have already resolved and part c and d tobe resolved. a. What is a genetic mutation? How do genetic mutations differ fromsomatic mutations? b. What are mutagens? Using examples, describe how chemical andphysical mutagens cause mutations. c. Briefly describe the significance of transposons in inducing d. Discuss the practical aspects of mutations.arrow_forwardDiagram the mechanism by which CRISPR-cas functions in the immune system of bacteria.arrow_forward
- During experimental RNAi, how does the researcher affect expression of a target gene? Group of answer choices Inject custom, lab-made double-stranded RNA into the specimen that will be targeted by the cell's existing Dicer to create siRNA complementary to the target gene's transcript. Inject lab-made Dicer proteins into the specimen which will cut up the target gene's transcript. Inject custom, lab-made siRNAs complementary to the target gene's transcript.arrow_forwardThe goal of most gene therapies is to insert a healthy copy of a gene into the genome. Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions. A. It has been shown that where the healthy gene integrates is heavily influenced by chromatin. Why? B. The same gene integrated into different locations in the genome will be expressed differently. Why? C. Over time the expression of an integrated gene will change, usually decreasing. Describe how you think the cell downregulates expression of an integrated gene.arrow_forwardWhich of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.arrow_forward
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