Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 21.5, Problem 3COMQ
During Western blotting, the primary antibody recognizes
a. the secondary antibody.
b. the protein of interest.
c. an mRNA of interest.
d. a specific fragment of chromosomal DNA.
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Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- Which of the following statements best describes the function of SDS in western blotting? Select one: A. It can solidify polyacrylamide gel. B. It allows the transfer of proteins from polyacrylamide gel to nitrocellulose membrane. C. It can form a colored product with proteins. D. It can linearize protein molecules. E. It gives proteins a net positive charge. O O O Oarrow_forwardmRNA vaccines are better than DNA vaccines because A. their sequences can be easily modified. B. they can be easily scaled up. C. they can be easily degraded. D. they are single-stranded molecules. E. they do not integrate into the host DNA. O O O Oarrow_forward3 4 Some antibodies can be passed from mother to child during breastfeeding. What is this type of immunity called? A. Artificial passive B. Natural active C. Artificial active D. Natural passive The diagram represents the production of the hormone insulin in laboratory. Human Insulin Production Human pancreas cell Bacterial DNA & Human insulin- producing gene 60 Bacterium DNA Plasmid DNA Introduction of recombinant DNA into a bacterial cell Plasmid DNA cut with restriction enzymes Recombinant DNA A. Directed mutation B. Gene cloning C. Synthetic transplant D. Acquired immunity Recombinant Bacterium OO Fermentation Tank Human insulin Recombinant bacteria multiplying and producing human insulin in fermentation tank Human insulin https://www.eduhk.hk/biotech/eng/classm/explain/health3.jpg Extraction & purificaion of human insulin Source: The production of human insulin by E. coli is an example of the application of which of the following?arrow_forward
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- Which one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0arrow_forwardAntigenic drift refers to: A.The reassortment of genomic segments from different viruses B.Cytotoxic T cells identifying viral infected host cells C.The accumulation of mutations in the viral genome due to an error prone polymerase D.Antigen presenting cells failure to recognize the influenza virusarrow_forwardSelect the best technique to answer the following question: Where does the transcription factor Nanog bind the rex1 promater? a. EMSA . b. Northern blot . c. DNAse footprinting . d. Bacterial two-hybrid.arrow_forward
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