Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21, Problem 24EQ
If you wanted to know if a protein was made during a particular stage of development, what technique would you choose?
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For what the first clinical gene therapy was given?
Briefly describe the creation of a transgenic mouse in your lab.
List all the materials needed to clone a mouse.
Place the following steps in the correct order:
_______________ Stimulate cell division
_______________ Deliver baby
_______________ Remove and discard the nucleus from the egg cell
_______________ Isolate donor cells from egg donor and germ cell donor
_______________ Transfer the somatic cell nucleus into the egg cell
_______________ Implant embryo into a surrogate mother
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- List all the materials needed to clone a mouse. Place the following steps in the correct order: _______________ Stimulate cell division _______________ Deliver baby _______________ Remove and discard the nucleus from the egg cell _______________ Isolate donor cells from egg donor and germ cell donor _______________ Transfer the somatic cell nucleus into the egg cell _______________ Implant embryo into a surrogate mother 27. There are two time gaps in the process of cloning. What are they? (ie. What do you have to wait for?) What color will the cloned mouse be? What is the name of this mouse?arrow_forwardwhen creating a transgenic animal Explain why you need to culture the E. coli bacteria on agar that has been infused with ampicillin.arrow_forwardYou’ve made your construct and placed it into E. coli! Congratulations, you have made a transgenic organism. Your investors will want to know about quality control. How will you check that the correct piece of DNA is in your vector? How will you check to make sure the gene is transcribed? How will you check to make sure that the GasP protein is made in E. coli? Your investors are concerned that the GasP protein might not be sufficiently produced under normal laboratory conditions. They suggest controlling the transcription of the gasP gene using a chemical that will “trigger” its transcription. What type of promoter could be used? What chemical will you use to control transcription? How does this method of control work?arrow_forward
- With the many high throughput experiments that are used in biomedical research, how are some ways to integrate those datasets using systems biology? For example, if you had a microarray dataset that annotated gene expression levels and a proteomics dataset that identifed protein interactions, how could you jointly use both datasets to identify markers for a disease? Explain each step.arrow_forwardWhat are Gene-knockout experiments?arrow_forwardHow did the private corporation Celera Genomics approach the sequencing of the human genome? What was the advantage of this approach?arrow_forward
- Ashanti Silva received the first gene therapy for the treatment of SCID. What do you think, would the combination of gene therapy and stem cell therapy be a better therapeutic option?arrow_forwardLet’s say, you want to deliver a gene into a cell and in your lab, there are lot of options available to do that. Describe five different options that you can use to deliver that gene.arrow_forwardAnswer the two parts of the question. a) Explain what gene therapy involves.b) Discuss how gene therapy can be used to treat/cure or prevent a disease and include in your answer any current challenges associated with its use.arrow_forward
- List all steps involved in a cloning workflow. Name and link the appropriate enzymes to the steps at which they are used.arrow_forwardI believe that there are many good things that can come out of people getting to design their baby’s genetic material. But there are also many bad things as well. From the article by Bio medical about the pros and cons of having a designer baby it states that a pro is that this type of engineering can “ might help prevent genetic diseases such as Alzheimer’s, Huntington’s Disease, down syndrome, Spinal Muscular Atrophy, and many others”. I think that it is great that we could get rid of Alzheimer’s due to how destructive it can be to the people that suffer it. But I think the other diseases that it can eliminate is horrible due to them making our world a more unique place such as people with autism, Down syndrome. By doing this it could eliminate the whole population of people with disabilities community and make everyone “normal”. Another bad that I found in the article Ethics of designer babies which states that a major flaw for these babies is “designer baby technologies suggest…arrow_forwardA student wants to observe how the environment of bacteria affects gene expression. How should the experiment be designed? A. exposing the same species of bacteria to the same carbohydrates in each sample B. exposing the same species of bacteria to different carbohydrates in each sample C. exposing different species of bacteria to the same carbohydrates in each sample D. exposing different species of bacteria to different carbohydrates in each samplearrow_forward
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