Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 21.1, Problem 5COMQ
A collection of recombinant vectors that carry fragments of chromosomal DNA is called
a. a genomic library.
b. a cDNA library.
c. a Northern blot.
d. either a or b.
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A collection of recombinant vectors that carry fragments of chromosomalDNA is calleda. a genomic library.b. a cDNA library.c. a Northern blot.d. either a or b.
When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
Which of the following statements about CRISPR-Cas9 is correct?
O A. It relies on the natural process of DNA repair.
B. Regardless of the DNA target, the sequence of the guide RNA is always the same.
C. Cas9 is only involved in recruitment of guide RNA to its target DNA.
D. It is not precise enough to make a single base pair edit.
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- The CRISPR-Cas9 system can be used to: a. sequence a genome in living cells b. edit a gene in living cells and organisms c. diagnose sickle-cell anemia d. identify a specific gene e. detect gene expression under different conditionsarrow_forwardIf you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know if the protein encoded by the cloned gene is expressed or not, which of the following methods is the right one to use? Select one: a. Northern blot b. Both Northern and Western blots c. Agarose gel with polyacrylamide d. Western blot e. Protein gel and northern blotarrow_forwardWhich of the following approaches you can use to locate the relative positions of genes on chromosomes? A. Recombination-based mapping B. In situ hybridization C. Antibody localization D. Yeast two hybrid O E. Site-directed mutagenesis F. Reporter gene reconstructsarrow_forward
- Which of the following methods use(s) a labeled nucleic acidprobe, such as a labeled fragment of DNA?a. Site-directed mutagenesis c. Western blottingb. Northern blotting d. Both a and barrow_forwardWhich one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0arrow_forwardAnswer the following questions about CRISPR below: A.What is a PAM sequence? B.How does the Cas-9/RNA sequence target a piece of DNA for destruction? C.How is the DNA repaired after being cut by CRISPR/Cas-9?arrow_forward
- Select the best technique to answer the following question: Where does the transcription factor Nanog bind the rex1 promater? a. EMSA . b. Northern blot . c. DNAse footprinting . d. Bacterial two-hybrid.arrow_forwardWhich is the BEST technique to use to answer this question? You wish to determine whether a point mutation is present in the sunscreen gene. a. Westem blot. b. Northern blot . c. Sanger DNA sequencing d. In situ hybridization. Part B. Which is the BEST technique to use to answer this question? You wish to determine where Sunshine protein is translated in a ladybug embryo. a. Immunofluorescencez b. Western blot . c. RT-PCR. d. Northern blot. e. in situ hybridization.arrow_forwardA.) Homologous recombination repair are prone to error and can cause loss of genome integrity. B.) Reversal and repairing of DNA strands damage is important in maintaining genome integrity. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forward
- What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forwardChoose the statement that best describes the outcome of transfecting cells with a plasmid that includes the genetic promoter of the hypothetical X protein in a plasmid coding for GFP. A.GFP will be expressed under the same conditions and in the same cells as the X protein. B.GFP combined with the X protein will be expressed in all transfected cells. C.PFM will not be expressed as it requires its own sponsor, the PFM sponsor, to be properly expressed. D.GFP will replace the X protein in transfected cells.arrow_forwardIn gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far apart on the bacterial chromosome. b. on different bacterial chromosomes. c. close together on the bacterial chromosome. d. on a plasmid.arrow_forward
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