The results of the northern blotting of the X-linked gene in X b Y male, X b X b female, and X B X b female mouse. Introduction: The technique in which the mRNA (messenger Ribonucleic acid) is detected using autoradiography is termed as northern blotting. The production of mRNA in varying cell types, organisms and different stages of development can be studied.

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Chapter 21, Problem 21EQ

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The results of the northern blotting of the X-linked gene in X^bY male, X^bX^b female, and X^BX^b female mouse.

Introduction:

The technique in which the mRNA (messenger Ribonucleic acid) is detected using autoradiography is termed as northern blotting. The production of mRNA in varying cell types, organisms and different stages of development can be studied.

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Let’s suppose an X-linked gene in mice exists as two alleles, whichwe will call B and b. X-chromosome inactivation, a process inwhich one X chromosome is turned off, occurs in the somatic cellsof female mammals . Allele B encodes an mRNA that is 900 nucleotides long, whereas allele b contains a small deletion that shortens the mRNA to a length of 825 nucleotides.Draw the expected Northern blot that will be obtained usingmRNA isolated from somatic tissue of the followingmice:Lane 1. mRNA from an XbY male mouseLane 2. mRNA from an XbXb female mouseLane 3. mRNA from an XBXb female mouse.Note: The sample taken from the female mouse is not from aclone of cells. It is from a tissue sample, like the one shown.

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