Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21.2, Problem 2COMQ
Summary Introduction
Introduction:
The PCR (polymerase chain reaction) technique is used to for amplification of DNA (deoxyribonucleic acid) and this is useful in making multiple copies of the same template strand. The template strand used is theDNAstrand. The reverse transcriptase PCR makes multiple copies of the DNA through the reverse transcription of the RNA (ribonucleic acid).
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Which of the following is true?
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In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore.
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Next generation sequencing is slow but accurate.
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Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- Which of the following reagents is NOT required for a PCR? a. DNA template b. DNA Polymerase c. All four dNTPs d. small amount of ddNTPs e. Pair of primersarrow_forwardThe image below represents the PCR results after electrophoresis on an agarose gel. One band is labelled as (i) and another is labelled as (ii). Which of the following descriptions below best describes the band labelled as (i)? a. Relaxed DNA b. Primers c. RNA d. Amplicon e. Digested DNA fragment f. None of the abovearrow_forwardIn reverse transcriptase PCR, the starting biological material isa. chromosomal DNA. c. proteins.b. mRNA. d. all of the above.arrow_forward
- Which of the following is not required for PCR? a. dNTPs b. bacterial plasmids c. carefully designed primers d. taq or other heat- resistant polymerases e. template DNAarrow_forwardPCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardWhat would be the effect of performing a RT-PCR with the following ingredients: an mRNA template, appropriate primers, dNTPs, heat-stable reverse transcriptase and human DNA polymerase? Select one: a. The PCR would occur, but with a high mutation rate b. The PCR reaction will not commence c. Non-specific PCR of random templates will occur d. The RNA template would be converted to DNA, but the DNA segment would not be amplified PCR would proceed normally е.arrow_forward
- Match the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forwarde. stop nucleotides; restriction enzymes 18. Choose the correct number of DNA molecules created after 6 cycles of PCR. a. 6 b. 16 c. 32 d. 64 c. 128 LICHC 53.000arrow_forwardWhich of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forward
- Which of the following is involved in recombinant DNA technology? Explain. MULTIPLE CHOICE. Choose only one: a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forwardWhich of the following is involved in recombinant DNA technology? Explain. a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forwardWhich among these can the expression of the gene of interest be increased via (multiple choice, choose one) A. PCR B. bacterial vectors C. PCR and bacterial vectors D. none of the abovearrow_forward
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