Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21.1, Problem 3COMQ
Which is the proper order of the following steps in a gene-cloning experiment involving vectors?
1. Add DNA ligase.
2. Incubate the chromosomal DNA and the vector DNA with a restriction enzyme.
3. Introduce the DNA into living cells.
4. Mix the chromosomal DNA and vector DNA together.
a. 1, 2, 3, 4 c. 2, 4, 1, 3
b. 2, 3, 1, 4 d. 1, 2, 4, 3
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What is the proper order of the following steps in a gene-cloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4b. 2, 3, 1, 4c. 2, 4, 1, 3d. 1, 2, 4, 3
1. How may recombinant DNA molecules be introduced into human cells?
a. by splicing the needed genes into a mammalian chromosome using restriction enzymes.
b. by adding plasmids to the mammalian cells.
c. by using engineered viruses as vectors.
d. by using a gene gun.
2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)
. 1 · | 2. L 3 | 4 | .
5 6 7
Direction: Encircle the letter of the correct answer .
1.
It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is
widely used as a gene cloning vector.
A. Cosmid B. Plasmid. C. Phagemid. D. Genome
2.
In plant genetic engineering, which of the following acts as vector?
B. Gene of interest
D. Ti-plasmid
А.
Agrobacterium lumefaciens.
Receipt plant cell.
Which of the following illustrates breeding?
A farmer choose a breed of cow for greater milk production
The use of bacteria in order to produce human insulin.
The insertion of cloned genes to plant cells.
All of the above.
3.
А.
В.
С.
D.
Being pest resistance is one of the traits that are being introduced to plants like corn and
eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is
inserted to plants?
A. Biolistic
C. Plasmid insertion by heat shock treatment
4.
B. Electroporation
D. All of these
5. A biotechnologist wants to enhance…
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.arrow_forwardGenetically engineered human insulin, human growth hormone, and human clotting factor VIII are made by:a. gel electrophoresis b. polymerase chain reactionc. transgenic bacteriad. DNA fingerprintingarrow_forwardPart A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forward
- In gene reflection, which is part of a designated vector that cannot be part of the original 1. plasmid? A. Foreign or transformed gene B. Ori site C. Multiple cloning site D. Resistance gene 2. Statement I: During ligation, a recombinant DNA technology have different meanings Statement II: Recombination can only be facilitated with DNA tools and genetic engineering. A. Both are correct. B. Both are false. C. Statement I is correct and Il is false. D. Statement I is incorrect and II is correct.arrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardWhich of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA polymerase c. gel electrophoresis d. high temperaturearrow_forward
- In 1996, a group of scientists from Scotland used a somatic cell of an adult sheep and fused it with an egg. They wanted to produce an identical cony of that sheep. The process through which a genetically identical cell or organism is produced is known as O A. cloning O B. carbon copying O C. xeroxing O D. vector analysisarrow_forward1. Scientists were able to develop transgenic bacteria that can be used for mercury remediation. This process involved combining genes for mercury resistance (so that the bacteria itself has protection) and mercury accumulation (thereby allowing the bacteria to sequester Hg for retrieval later on). They probably used the following techniques in creating the transgenic bacteria EXCEPT a. plasmids b. DNA ligase c. DNA polymerase d. restriction enzymes 2. Consider these two parents (Mom and Dad) and their son, Calvin. (see pic attached). Which two members of this family would show the greatest difference in their DNA profile?arrow_forwardOne of the following can not be used in therapeutic cloning Select one: a. Embryonic b. Blood cells c. Adult stem cells d. Umbilical cordarrow_forward
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