Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 19TYK
Summary Introduction
Introduction: In recombinant DNA technology (rDNA technology), various tools and techniques are used to identify, amplify, and diagnose the expression of target genes. Various markers are used to locate the position of the particular gene sequences. These markers are usually radiolabeled or immune-labeled and have complementary sequence to the target genes.
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How can you detect 1 small, very specific, piece of DNA (eg: a gene), among THOUSANDS of other pieces or sequences of DNA from nuclear chromosomes?
a. DNA hybridization
b. RNA hybridization
c. Use of a radioactive or fluorescent probe
d. Colony blotting and hybridization
e. Synthetic primers and PCR
f. Any of the above methods
Which is the BEST technique to use to answer this question?
You wish to determine whether a point mutation is present in the sunscreen gene.
a. Westem blot.
b. Northern blot .
c. Sanger DNA sequencing
d. In situ hybridization.
Part B. Which is the BEST technique to use to answer this question? You wish to determine where Sunshine protein is translated in a ladybug embryo.
a. Immunofluorescencez
b. Western blot .
c. RT-PCR.
d. Northern blot.
e. in situ hybridization.
For each situation, write the letter of the technique that would be most helpful;
A. DNA editing
A doctor wants to know if a patient has an inherited
using CRISPR
B. DNA replication
using PCR
C. DNA analysis
through genetic
testing
D. DNA insertion
16.
disorder.
I
A scientist needs many copies of a gene to conduct an
17.
experiment.
A genetic engineer wants to replace a defective copy of
a gene with a functional copy in a chromosome.
18.
into bacteria as a
plasmid
A medical researcher needs many copies of a protein
19.
(insulin) to be produced to use in a medical treatment.
A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one
produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter
one letter in each blanks (19 & 20) to correctly complete the sentences.
ninate Education TM, Inc.
Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardWhat is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forwardWhich of the following is involved in recombinant DNA technology? Explain. a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forward
- Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardWhat are the advantages of real-time PCR over microscopy for diagnosing malaria? a. Giemsa stain is not required for real-time PCR b. It allows for species identification and quantification of malaria-causing plasmodium at lower blood concentration with greater sensitivity and specificity. c. Real-time PCR doesn't require the use of a microscope which is deemed too expensive. d. Real-time PCR has a hiher sensitivity for the main malaria-causing plasmodium, P. falciparum, than microscopy techniques. Thank you!!!arrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forward
- When bacteria are consumed by macrophages, the macrophages give off chemokines that recruit other immune cells. The chemokines serve as ligands for G-protein coupled receptors. Which of the following is NOT true of G-PCRs? a. G-PCRs require GTP to activate a G-protein b. G-PCRs bind the ligand extra-cellularly and undergo an allosteric change c. The receptor is a G-protein d. The G-protein separates into multiple parts that can activate different pathways.arrow_forwardWhen constructing a recombinant DNA molecule, a marker gene is used to: a. give the organism a new trait, such as insect resistance b. Identify whether the transformed organism contains the recombinant DNA c. replicate (copy) the gene of interest d. Introduce the recombinant DNA into an organism e. cut short sequences of DNAarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organismarrow_forward
- PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forwardGenetically engineered human insulin, human growth hormone, and human clotting factor VIII are made by:a. gel electrophoresis b. polymerase chain reactionc. transgenic bacteriad. DNA fingerprintingarrow_forward
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