Study Guide for Campbell Biology
Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 20TYK
Summary Introduction

Introduction: The restriction enzymes are present in bacteria and in other prokaryotes. The restriction enzymes recognize short nucleotide sequences called restriction sites. The restriction sites are usually symmetrical.

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The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted with hemophilia. The man with blackened symbol (III-1) has hemophilia. To help with genetic diagnosis, a probe that detects an RFLP (restriction fragment length polymorphism) on the X chromosome is used. This probe detects either a 7 kb restriction enzyme fragment or 3 kb and 4 kb restriction enzyme fragments. The RFLP pattern for all the members of the pedigree is shown. The recombination distance between the RFLP and the hemophilia locus is 40%. What is the chance that their unborn child (III-7) is affected? 4 kb 3 kb Select one: a. 10% b. 20% C. 40% d. 60% e. 80% f. 90% g. None of these above II II
Genomic DNA from a family where sickle-cell disease is known to be hereditary, is digested with the restriction enzyme MstII and run in a Southern Blot. The blot is hybridised with two different 0.6 kb probes, both probes (indicated in red in the diagram below) are specific for the β-globin gene (indicated as grey arrow on the diagram below). The normal wild-type βA allele contains an MstII restriction site indicated with the asterisk (*) in the diagram below; in the mutated sickle-cell βS allele this restriction site has been lost. What size bands would you expect to see on the Southern blots using probe 1 and probe 2 for an individual with sickle cell disease (have 2 βS alleles)?     Probe 1 Probe 2 (a) 0.6kb 0.6kb and 1.2kb (b) 0.6kb and 1.8kb 0.6kb, 1.2kb and 1.8kb (c) 1.2kb 0.6kb (d) 1.8kb 1.8kb   a. (a)   b. (b)   c. (c)   d. (d)
STR markers: are point mutations detectable by DNA sequencing are variations in the number of repeats of very short DNA motifs (2-10 nucleotides) □have high polymorphism are mutations leading to proteins or blood groups that can be differentiated by antigenic testing from a blood sample ☐have low polymorphism no correct answer are changes of a few nucleotides leading to the absence or presence of a site recognized by a restriction enzyme are variations in the number of repeats of medium-sized DNA motifs (10-100 nucleotides) can be located in coding sequences are located exclusively on autosomes
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Serology 101: Testing for IgG and IgM antibodies; Author: Beckman Coulter Dx;https://www.youtube.com/watch?v=LtqKB-qpJrs;License: Standard youtube license