What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases?   a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d.   The DNA band detected by Southern blot is different from that by Northern blot

One would be correct in assuming that the restriction sites present in the multiplecloning site of the pUC18 plasmid will

which of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase  d. a growth medium with carefully selected ingredients that take advantage of selectable markers.  e. none of the above.

When bacteria are consumed by macrophages, the macrophages give off chemokines that recruit other immune cells. The chemokines serve as ligands for G-protein coupled receptors. Which of the following is NOT true of G-PCRs?       a.   G-PCRs require GTP to activate a G-protein       b.    G-PCRs bind the ligand extra-cellularly and undergo an allosteric change       c.    The receptor is a G-protein       d.    The G-protein separates into multiple parts that can activate different pathways.

If a F plasmid does not have the gene encoding TraJ, this F plasmid would Select one: a. become more active for conjugation O b. become more active for transformation. O c. become less active for transformation. O d. become less active for conjugation

Which of the following natural cellular process does PCR use to amplify a target DNA? A. Gene Expression B. DNA symthesis C. Transcription of DNA D. Translation

Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.

A contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.

What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?

Select the best technique to answer the following question: Where does the transcription factor Nanog bind the rex1 promater? a. EMSA . b. Northern blot . c. DNAse footprinting . d. Bacterial two-hybrid.