Study Guide for Campbell Biology
Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 17TYK
Summary Introduction

Introduction: Recombinant DNA (rDNA) technology involves the modification of an organism’s gene by inserting a gene of interest into it, so that the organism produces large amounts of required protein product. This involves many enzymes like restriction enzymes to splice the nucleotide at specific points, and ligases to join the sticky ends. This rDNA is inserted into a plasmid, which is later introduced into the target cell of the animal by infecting it with a suitable bacterium.

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Write the advantages and disadvantages of applying such application in the DNA of an organism. a. Drug delivery systems that are based on bacterial or viral host. b. Producing genetically modified organisms to enhance food production. c. Combining two or more genes in one organism to enhance its traits. d. DNA manipulation of certain species to produce organs for harvesting.
It has been suggested that it would make the study of human diseases easier if cloned transgenic animals were produced that carried faulty versions of human genes (e.g., the gene that causes cystic fibrosis). a. Why would such animals be useful in medical research? : b. What ethical questions are raised by the creation of such transgenic animals?
2) Transgenic animal refers to an organism whose genome has been altered by the transfer of a gene or genes from another species or breed. Cows, pigs and goats had been modified especially for pharmaceutical purposes. Explain three (3) most used transfection technology for the creation of transgenic animals.
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