Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Textbook Question
Chapter 20, Problem 2SYK
Fill in the table on the previous page on the basic tools used in DNA technology.
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Label the image below with ALL the pertinent information related to gene cloning. Make sure you use the following terms: bacterial plasmid, the gene of interest, recombinant plasmid, restriction enzymes, DNA ligase, insert plasmid into bacteria, cloning of plasmid in culture, etc.)
Draw the PCR machine
with marking in detail
Brenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break?
A. Obtaining and handling DNA sample without wearing googles or gloves
B. Improper use of human DNA samples
C. Violating Patient Confidentiality
D. Disposing of bio hazardous material in a regular trash
Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- Select the statement below that is FALSE regarding DNA and DNA Fingerprinting: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. DNA fingerprints can be used to link remains to a particular person. a b Restriction enzymes cut molecules into fragments of identical lengths. Restriction enzymes cut molecules of DNA into a unique set of fragment length determined by the genetic code. d Subunits of DNA are called bases and include (adenine/guanine/ cytosine/ and thymine)arrow_forwardFind a recently developed, automated DNA extraction technique/equipment andexplain it in detailarrow_forwardA restriction enzyme digests DNA into fragments. Name the technique used to check the progression of this enzyme and separate DNA fragments.arrow_forward
- Can you please check my answer and make sure it is correct. Question: Describe the two roles primers play in PCR. Answer: Primers are short sequences of single stranded DNA that mark both the ends of the target sequence to be amplified during PCR. One primer attaches to the top strand at one end (forward primer) and the other one attaches to the bottom strand at the other end of the segment (reverse primer). They are made specifically to attach to these sections of DNA because the nucleotides of the primers are complementary to the target sequence. Other than marking the starting and end points for PCR, primers are also necessary for the process because without them, DNA polymerase wouldn’t be able to synthesize the complementary strand of DNA because it can only add nucleotides to an existing piece.arrow_forwardthey decided to check which bacterial colonies contains the recombinant gene W by PCR. they designed one set of primers, gene W forward, and gene W reverse, to amplify gene W. Complete the PCR profile that will be used below.arrow_forwardConsider two transgenic organisms (microorganism, plant, animal). Diagram the procedure on how the two transgenic organisms were created. Indicate the gene introduced and the procedure to create the said transgenic organism.arrow_forward
- schematic diagram for the procedure in doing DNA extraction, isolation and quantificationarrow_forwardIdentify if the following statements are true or false. Quantitative PCR is also known as the real-time PCR Real-time PCR method uses fluorescent dyes such as acridine orangearrow_forwardName four reagents used during a PCR reactionarrow_forward
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DNA Use In Forensic Science; Author: DeBacco University;https://www.youtube.com/watch?v=2YIG3lUP-74;License: Standard YouTube License, CC-BY
Analysing forensic evidence | The Laboratory; Author: Wellcome Collection;https://www.youtube.com/watch?v=68Y-OamcTJ8;License: Standard YouTube License, CC-BY