Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 9TYK
Summary Introduction
Introduction: Recombinant DNA technology is a process of combining the DNA sequence of two or more organisms in an artificial manner to acquire the desired protein product. This is brought about by inserting the gene of interest into a plasmid. This is known as recombinant plasmid. This recombinant plasmid is then introduced into a bacterium to produce clone of cells having the gene of interest.
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Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease
b. virus receptors
c. a gene for antibiotic resistance
d. reverse transcriptase
Choose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptase
What repair system will have an issue functioning properly if DNA glycosylase loses its function?
A. Photoreactivation repair
B. Base excision repair
C. Nucleotide excision repair
D. SOS repair
Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- A mutation in which of these proteins will lead to increased mutations in all daughter cells? A. helicase B. topoisomerase C. ligase D. DNA polymerasearrow_forwardDNA polymerases that corrects errors and mismatches in DNA is called? A. Post replication repair B. Base excision repair C. Nucleotide excision repair D. Proofreadingarrow_forwardWhat is used to cut the DNA in panels 1 and 2? a. Restriction enzymes b. DNA polymerases c. DNA ligases d. RNA polymerases e. Reverse transcriptasearrow_forward
- Which protein directs the recombination exchange with a DNA that has failed a complete DNA replication? A. UvrA B. RecB C. MutC D. RecAarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardWhat is the correct sequence action of proteins during the process of DNA replication? A. DNA polymerase, helicase, primase, ligase, SSB B. helicase, SSB, primase, ligase, DNA polymerase C. ligase, helicase, SSB, primase, DNA polymerase D. helicase, SSB, primase, DNA polymerase, ligasearrow_forward
- What is meant by the term DNA replication? a. synthesis of nucleotides b. cell division c. interpretation of the genetic code d. the exact copying of the DNA code into two new moleculesarrow_forwardWhich of these is a polymer made of nucleotides? a. RNA polymerase b. CAS9 c. restriction enzyme d. mRNAarrow_forwardMatch the process to the step in producing a transgenic plant using the Ti plsmid method. 45. A gene is isolated and the process starts. 46. The recombinant DNA has this so it can be found later. 47. The plasmid enters here. 48. A. tumefaciens infects target organism. 49. The selectable marker allows transgenic plants to survive antibiotic. 50. Transgenic plant cells are isolated. 51. Transgenic plants are prepared for life in the soil. Kanamycin is added to culture. b. The isolated transgenic cells are grown into multicellular organisms. C. Ti plasmid picks up gene of interest. d. Bacterium Agrobacterium tumefaciens takes this up. A selectable marker makes the cell resistant to kanamycin. a. e. f. Antibiotics kill any remaining bacteria. g. The host plant is selected and exposed to the new DNA.arrow_forward
- Which enzyme adds RNA nucleotides to mark the starting point for the synthesis of a new DNA strand? A. DNA polymerase I B. DNA polymerase III C. primase D. helicase The spontaneous loss of amino groups from adenine in dna results in hypoxanthine an uncommon base opposite thymine. what combination of proteins could repair such damage? A. telomerase, primase, DNA polymerase B. nuclease, DNA polymerase, DNA ligase C. telomerase, helicase, single-strand binding protein D. DNA ligase, replication fork proteins, adenylyl cyclase A section of DNA has the following sequence of nitrogenous bases: CGATTACAG. Which of the following sequence would be produced as a result of transcription? A. CGTUUTCTG B. GCTAATGTC C. CGAUUACAG D. GUAAUGUCarrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? a.DNA ligase seals any gaps remaining between bases of new DNA. b.Primers base-pair with the exposed single DNA strands. c.Repair enzymes correct potential mutations in the DNA sequence. d.Enzymes unwind and separate the two strands of DNA.arrow_forwardWhich enzyme is responsible for the synthesis of pre-mRNA? A. RNA polymerase I B. RNA polymerase II C. RNA polymerase III D. RNA polymerasearrow_forward
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